-
Immunogen
Synthetic peptide of human BRAF (AA447-461)
-
Antibody s target
Human BRAF
-
Antibody s full description
Rabbit Anti-Human BRAF Polyclonal
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
94 kDa B raf protein antibody, B raf 1 antibody, B Raf proto oncogene serine threonine protein kinase antibody, B RAF1 antibody, B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody, BRAF 1 antibody, BRAF antibody, BRAF_HUMAN antibody, BRAF1 antibody, Murine sarcoma viral (v-raf) oncogene homolog B1 antibody, NS7 antibody, p94 antibody, Proto-oncogene B-Raf antibody, Proto-oncogene c-Rmil antibody, RAFB 1 antibody, RAFB1 antibody, RMIL antibody, Serine/threonine-protein kinase B-raf antibody, v raf murine sarcoma viral oncogene homolog B antibody
-
Verified applications
WB, AM
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse
-
Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects 84.491 kDa.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:250 dilution of SPC-1110 was sufficient for detection of BRAF in 10 µg of human liver lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Nucleus, Cytoplasm, Cell membrane
-
Tissue specificity
Brain and testis
-
Scientific context
BRAF is a serine/threonine kinase that participates in the RAS>RAF>MEK>ERK/MAPK pathway, which is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Ras isoforms are inactive when bound to GDP, but when they are bound to GTP they become active and promote phosphorylation and activation of B-Raf. BRAF is expressed in most tissues with high expression in neuronal tissue. Several genes have been found to be activated by this pathway, among them, cyclins D1, D2 and D3, VEGF, c-myc, b3-integrin and mdm2. BRAF is frequently mutated in human cancers (e.g. sarcomas, colorectal adenocarcinomas, metastatic melanomas, ovarian serous carcinomas, pilocytic astrocytomas); out of 153,189 human tumours, 20.2% featured RafB mutations primarily with the V600E substitutions. This mutation results in constitutive and elevated kinase catalytic activity.
-
Bibliography
Refer to PubMed
-
Released date
29/Aug/2016
-
NCBI number
NP_004324
-
Gene number
673
-
Protein number
P15056
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
Representative figure link
No Data Available
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Conjugation
not conjugated
-
Gene target
-
Gene symbol
BRAF
-
Short name
Rabbit Anti- BRAF Polyclonal: Unconjugated
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
Immunoglobulin
-
Label
Unconjugated
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman v-raf murine sarcoma viral oncogene homolog B1 polyclonal: nonconjugated
-
Alternative technique
polyclonals, rabbit-anti, antibodies
-
Alternative to gene target
v-raf murine sarcoma viral oncogene homolog B1, B-RAF1 and BRAF1 and NS7 and RAFB1, BRAF and IDBG-44351 and ENSG00000157764 and 673, transferase activity, nuclei, Braf and IDBG-138657 and ENSMUSG00000002413 and 109880, BRAF and IDBG-636391 and ENSBTAG00000021761 and
-
