Phospho-A-RAF (Thr214) Antibody

Type

primary antibody
Shipping

Ice bag
Antigen

Phospho-A-RAF (Thr214)
Reactivity

Human,Mouse,Rat
Clonality

Polyclonal
Application

WB,ELISA
Application_advice

WB 1:500-1:2000
Epitope

Phospho Thr214
Immunogen

A synthesized peptide derived from human A-RAF around the phosphorylation site of Thr214.
Purification

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Format

Liquid
Concentration

1mg/ml
Fragment

Fab fragment
Storage_Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Specificity

Phospho-A-RAF (Thr214) Antibody detects endogenous levels of A-RAF only when phosphorylated at Thr214.
GendID

369
Gene

ARAF
GeneAlias

ARAF ARAF1 PKS PKS2
Unigene

Hs.446641;
Swiss Prot

P10398
Weight

68kDa
Datasheet
Msds
Properties

If you buy Antibodies supplied by Affinity Biosciences they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation

anticorps

Gene target

Phospho-A-RAF Thr214
Gene symbol

RAF1, ARAF, HLA-A, IGHV5-10-1, CIDEA, RPA1, CDC37P1, OR1Q1, LINC01139
Short name

Phospho-A-RAF (Thr214) Antibody
Technique

Antibody, antibodies against human proteins, antibodies for
Host

Rabbit
Isotype

IgG
Alternative name

phosphorilated-A-RAF (Thr214) (antibody to-)
Alternative technique

antibodies

Identity

HGNC:5661
Gene

IGHV5-10-1
Long gene name

immunoglobulin heavy variable 5-10-1
Synonyms gene
- IGHV5-A
Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
Synonyms
- IGHV5-a
GenBank acession
- X92227
Locus

14q32.33
Discovery year

2000-04-04
Entrez gene record

28386
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000191143

Identity

HGNC:10289
Gene

RPA1
Long gene name

replication protein A1
Synonyms gene name
- replication protein A1 (70kD)
- replication protein A1, 70kDa
Synonyms
- REPA1
- RPA70
- HSSB
- RF-A
- RP-A
GenBank acession
- M63488
Locus

17p13.3
Discovery year

1993-12-14
Entrez gene record

6117
Pubmed identfication
- 8454588
RefSeq identity
- NM_002945
Classification
- Nucleotide excision repair
VEGA ID

OTTHUMG00000090579

Identity

HGNC:34406
Gene

CDC37P1
Long gene name

cell division cycle 37 pseudogene 1
Synonyms gene name
- cell division cycle 37 homolog (S. cerevisiae) pseudogene 1
Synonyms
- A-761H5.2
Locus

16p11.2
Discovery year

2009-01-29
Entrez gene record

390688
Pubmed identfication
- 15616553
RefSeq identity
- NG_009463
VEGA ID

OTTHUMG00000175739

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data