Caspase-12 Antibody

• Catalog number
  3282-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Caspase -12
• Target Alternative Name
  CASP12 , CASP12P1
• Antibody Type
  Polyclonal
• Species Reactivities
  Mouse, rat
• Immunogen Sequence
  Synthetic peptide corresponding to residues surrounding amino acid 310 of mouse caspase-12
• Accession
  NM_009808
• Gene Id
  12364
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.2 mg/ml
• Formulation
  100 µg (0.2 mg/ml) affinity purified rabbit anti-caspase-12 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blotting (0.5-4 µg/ml). It detects procaspase-12 (55 kDa) and cleavage caspase-12 (36 kDa) on SDS-PAGE immunoblots. However, the optimal conditions should be determined individually.
• Description
  Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains two pathways, in which the key apoptotic proteases caspase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is located to the ER, but not to the cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium hemeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is localized to the ER with several proteins that are involved in Alzheimer’s disease including gamma-secretase presenilin and β-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-β. Caspase-12 is ubiquitously expressed in mouse tissues.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• French translation
  anticorps

• Gene target
  Caspase-12  
• Gene symbol
  CASP12, AVEN, NLRC4
• Short name
  Caspase-12 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  caspase-12 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:19004
• Gene
  CASP12
• Long gene name
  caspase 12 (gene/pseudogene)
• Synonyms gene
  • CASP12P1
• Synonyms gene name
  • caspase 12 pseudogene 1
  • caspase 12
• GenBank acession
  • AF464191
• Locus
  11q22.3
• Discovery year
  2002-07-25
• Entrez gene record
  100506742
• Pubmed identfication
  • 12054529
  • 9038361
  • 16917906
  • 16532395
• RefSeq identity
  • NM_001191016
• Classification
  • Caspases
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000154965

Gene info

• Identity
  HGNC:13509
• Gene
  AVEN
• Long gene name
  apoptosis and caspase activation inhibitor
• Synonyms
  • PDCD12
• Synonyms name
  • cell death regulator aven
  • programmed cell death 12
• GenBank acession
  • AF283508
• Locus
  15q14
• Discovery year
  2002-09-17
• Entrez gene record
  57099
• Pubmed identfication
  • 10949025
• RefSeq identity
  • NM_020371
• VEGA ID
  OTTHUMG00000129371

Gene info

• Identity
  HGNC:16412
• Gene
  NLRC4
• Long gene name
  NLR family CARD domain containing 4
• Synonyms gene
  • CARD12
• Synonyms gene name
  • caspase recruitment domain family, member 12
• Synonyms
  • CLAN1
  • ipaf
  • CLANA
  • CLANB
  • CLANC
  • CLAND
  • CLR2.1
  • CLAN
• Synonyms name
  • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4
  • NOD-like receptor C4
• GenBank acession
  • AF376061
• Locus
  2p22.3
• Discovery year
  2002-09-19
• Entrez gene record
  58484
• Pubmed identfication
  • 11374873
• RefSeq identity
  • NM_021209
• Classification
  • NLR family
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000152107
• Locus Specific Databases
  • LRG_1317

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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