Hi-Bind™ Cibacron Blue-Agarose Beads
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Catalog number
7923-10
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Price
Please ask
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Size
10 ml
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Description
High binding beads for removal of albumin and numerous other proteins from multiple sample types.. Cibacron Blue has been shown to bind dehydrogenases, kinases, serum albumin, interferons, several plasma proteins, and a variety of other proteins. It is a popular ligand for dye-ligand affinity chromatography. Cibacron Blue-Agarose beads are prepared by covalent conjugation of Cibacron Blue 3G-A to cross-linked (4%) Agarose beads by a proprietary method. The beads can be used as a purification matrix for a variety of Cibacron Blue dye-binding proteins. In addition, they are useful for enrichment of low abundant non-Cibacron Blue binding proteins.
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Product Highlights
FORMULATION: Provided as 50% aqueous slurry containing 20% ethanol. Ligand Density: 8-10 µmol Cibacron Blue 3G-A per ml of drained gel/resin. BINDING CAPACITY: The binding capacity of the Hi-Bind™ Cibacron Blue 3G-A-Agarose beads is >18 mg of Human Serum Albumin per ml of the drained gel/resin. APPLICATIONS: BioVision’s Hi-Bind™ Cibacron Blue-Agarose beads can be used as an affinity purification matrix of variety of proteins for subsequent ELISA, immunoassays and other downstream analyses such as gel electrophoresis, functional assays, etc.
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Storage Temp
+4°C
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Shipping
gel pack
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Shelf Life
12 months
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Notes
For research use only. Not to be used for human or animal treatment or consumption.
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Properties
The purest agarose was used in the production of Hi-Bind™ Cibacron -Agarose by Biovision. Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.
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Conjugation
Agarose, Blue Substrate
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Gene target
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Short name
Hi-Bind™ Cibacron -Agarose
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Technique
Agarose, agaroses, All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet.
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Label
Blue
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Alternative name
Hi-Bind™ Cibacron Blue-molecular sieve particles
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Alternative technique
separation
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MeSH Data
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Name
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Concept
Scope note:
Electrophoresis in which agar or agarose gel is used as the diffusion medium.
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Tree numbers
- E05.196.401.153
- E05.301.300.100
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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