FORMULATION: Provided as 50% aqueous slurry containing 20% ethanol. Ligand Density: 8-10 µmol Cibacron Blue 3G-A per ml of drained gel/resin. BINDING CAPACITY: The binding capacity of the Hi-Bind™ Cibacron Blue 3G-A-Agarose beads is >18 mg of Human Serum Albumin per ml of the drained gel/resin. APPLICATIONS: BioVision’s Hi-Bind™ Cibacron Blue-Agarose beads can be used as an affinity purification matrix of variety of proteins for subsequent ELISA, immunoassays and other downstream analyses such as gel electrophoresis, functional assays, etc.
Cibacron Blue has been shown to bind dehydrogenases, kinases, serum albumin, interferons, several plasma proteins, and a variety of other proteins. It is a popular ligand for dye-ligand affinity chromatography. BioVision’s Cibacron Blue-Agarose beads are prepared by covalent conjugation of Cibacron Blue 3G-A to cross-linked (4%) Agarose beads by a proprietary method. The beads can be used as a purification matrix for a variety of Cibacron Blue dye-binding proteins. In addition, they are useful for enrichment of low abundant non-Cibacron Blue binding proteins.
Maximum time for storage
High binding beads for removal of albumin and numerous other proteins from multiple sample types.