Rabbit Anti-Human Beclin 2 Polyclonal: Biotin
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Catalog numberSPC-602D-BI
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Price:
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Size100 µg
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ImmunogenSynthetic peptide from the mid-protein of human Beclin 2
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Antibody s targetHuman Beclin 2
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Antibody s full descriptionRabbit Anti-Human Beclin 2 Polyclonal
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Primary research fieldsCell Signaling, Epigenetics and Nuclear Signalling, Transcription, Cancer, Tumor suppressors, Cancer, Autophagy, Cancer, Cancer Metabolism
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameBECN2 Antibody, Beclin-2 Antibody, Beclin-1 like protein 1 Antibody
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Verified applicationsWB, ICC/IF
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Raised inRabbit
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Antibody s reactivityHuman, Rat
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Antibody s recommended dilutions for useWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityPredicted molecular weight at ~48.1kDa. Observed bands in the 50-55kDa range, varied by lysates.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-602 was sufficient for detection of Beclin2 on rat liver lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextBeclin 2 is a novel coiled-coil protein related to the autophagic Beclin 1 protein. It is thought to interact with Bcl-2, an anti-apoptotic protein, and is believed to function in autophagy. It has alson been found to mediate G protein-coupled receptro (GPCR) degradation (1).
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Bibliography1. He C., et al. (2013) Cell. 154(5): 1085-1099.
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Released date1/Oct/2015
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NCBI numberNP_001277622.1
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Gene number441925
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Protein numberA8MW95
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-602). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-602) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 2 Antibody (D) Composite. | Western blot analysis of Human 293Trap cell lysates showing detection of ~48.1 kDa Beclin2 protein using Rabbit Anti-Beclin2 Polyclonal Antibody (SPC-602). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin2 Polyclonal Antibody (SPC-602) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.1 kDa. | Western blot analysis of Rat Liver showing detection of ~48kDa Beclin-2 protein using Rabbit Anti-Beclin-2 Polyclonal Antibody (SPC-602). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin-2 Polyclonal Antibody (SPC-602) at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~48kDa. Rabbit Anti-Beclin 2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-602) | Rabbit Anti-Beclin 2 Antibody used in Western blot (WB) on 293Trap cell lysates (SPC-602) | Rabbit Anti-Beclin-2 Antibody used in Western blot (WB) on Liver (SPC-602)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationBiotinylated
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolBECN2, BECN1P2
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Short nameRabbit Anti- Beclin 2 Polyclonal:
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelBiotin
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman Beclin 2 polyclonal: biotinilated
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene namebeclin 2
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Synonyms gene
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Synonyms gene name
- beclin 1, autophagy related, pseudogene 1
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Locus
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Discovery year2010-06-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeclin 1 pseudogene 2
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Synonyms gene name
- beclin 1, autophagy related, pseudogene 2
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Locus
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Discovery year2014-10-03
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data