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Rabbit Anti Human Beclin 2 Polyclonal Biotin

Rabbit Anti Human Beclin 2 Polyclonal Biotin is available 2 times from Stressmark antibodies labs

SPC-602D-BI | Rabbit Anti-Human Beclin 2 Polyclonal: Biotin size: 100 µg | 446.88 USD

Immunogen Synthetic peptide from the mid-protein of human Beclin 2
Antibody's other name BECN2 Antibody, Beclin-2 Antibody, Beclin-1 like protein 1 Antibody
Antibody's specificity Predicted molecular weight at ~48.1kDa. Observed bands in the 50-55kDa range, varied by lysates.
Certificate of analysis A 1:1000 dilution of SPC-602 was sufficient for detection of Beclin2 on rat liver lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Tissue specificity See included datasheet or contact our support service.
Released date 1/Oct/2015
Representative figure linkmore | details | see here
Representative figure legend Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-602). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (SPC-602) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 2 Antibody (D) Composite. | Western blot analysis of Human 293Trap cell lysates showing detection of ~48.1 kDa Beclin2 protein using Rabbit Anti-Beclin2 Polyclonal Antibody (SPC-602). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin2 Polyclonal Antibody (SPC-602) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.1 kDa. | Western blot analysis of Rat Liver showing detection of ~48kDa Beclin-2 protein using Rabbit Anti-Beclin-2 Polyclonal Antibody (SPC-602). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin-2 Polyclonal Antibody (SPC-602) at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~48kDa. Rabbit Anti-Beclin 2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-602) | Rabbit Anti-Beclin 2 Antibody used in Western blot (WB) on 293Trap cell lysates (SPC-602) | Rabbit Anti-Beclin-2 Antibody used in Western blot (WB) on Liver (SPC-602)
MeSH Data
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Product images
    #Human#.jpg

SPC-603D-BI | Rabbit Anti-Human Beclin 2 Polyclonal: Biotin size: 100 µg | 446.88 USD

Loading
PubMed number Refer to PubMed
Net weight (g) 0.1
Recommended buffer for storage PBS, 50% glycerol, 0.09% sodium azide
Antibody's reactivity Human, Rat
Warning information Non-hazardous
Raised in Rabbit
NCBI number NP_001277622.1
Gene number 441925
Protein number A8MW95
Antibody's recommended dilutions for use WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Tested applications To be tested
Primary research fields Cell Signaling, Epigenetics and Nuclear Signalling, Transcription, Cancer, Tumor suppressors, Cancer, Autophagy, Cancer, Cancer Metabolism
Antibody's datasheet Contact our support service to receive datasheet or other technical documentation.
Total weight (kg) 1.4
Stock availabilit In Stock
Scientific context Beclin 2 is a novel coiled-coil protein related to the autophagic Beclin 1 protein. It is thought to interact with Bcl-2, an anti-apoptotic protein, and is believed to function in autophagy. It has alson been found to mediate G protein-coupled receptro (GPCR) degradation (1).
Antibody's target Human Beclin 2
Antibody's full description Rabbit Anti-Human Beclin 2 Polyclonal
Country of production Canada
Antibody's purified from Peptide Affinity Purified
Antibody's concentration 1 mg/ml
Tested reactivity To be tested
Antibody in cell Cytoplasm
Antibody's category Polyclonal Antibodies
Bibliography 1. He C., et al. (2013) Cell. 154(5): 1085-1099.
Verified applications WB, ICC/IF
Storage recommendations -20°C
Shipping recommendations Blue Ice or 4°C
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test StressMark antibodies supplies antibodies that are for research of human proteins.
Properties Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation Biotinylated
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name Oryctolagus cuniculus
Gene targetBeclin
Short name Rabbit Anti- Beclin 2 Polyclonal:
Technique anti-Human, Polyclonal, Rabbit, anti
Host Rabbit
Label Biotin
Species Human
Alternative name production species: rabbit antibody to-H. sapiens Beclin 2 polyclonal: biotinilated
Alternative technique polyclonals
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