Endothelin 1 (EDN1) Polyclonal Antibody (Pig), FITC
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Catalog number
PAA482Po01-20ul-FITC
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Price
Please ask
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Size
20ul
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Description
A Rabbit polyclonal antibody against Pig Endothelin 1 (EDN1). This antibody is labeled with FITC.
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Specifications
Host: Rabbit; Species Reactivity: Pig; Clonality: polyclonal; Tested applications: WB; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_information
Sequence of the immunogen: EDN1 (Cys53~His203); Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Pigs and the smaller guinea pigs are frequent used as models for humans. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Group
Polyclonals and antibodies
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French translation
anticorps
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Gene target
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Gene symbol
EDN1
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Short name
Endothelin 1 (EDN1) Polyclonal Antibody ( ), FITC
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Technique
Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
FITC
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Species
Pig, Pigs and Guinea pigs
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Alternative name
Endothelin 1 (endothelin 1) polyclonal (antibody to-) (swine), fluorecein
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Alternative technique
polyclonals, antibodies, fluorescine
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Alternative to gene target
endothelin 1, ARCND3 and ET1 and HDLCQ7 and PPET1 and QME, EDN1 and IDBG-61847 and ENSG00000078401 and 1906, endothelin B receptor binding, Extracellular, Edn1 and IDBG-147056 and ENSMUSG00000021367 and 13614, EDN1 and IDBG-636110 and ENSBTAG00000008096 and 281137
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Gene info
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Identity
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Gene
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Long gene name
endothelin 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1989-04-06
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Entrez gene record
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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