5-Ethynyl-2'-deoxyuridine

Alternative_name

2′-Deoxy-5-ethynyluridine; 5-EdU
Description

A novel agent for fast and sensitive detection of DNA synthesis in vivo. 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. May be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis. EdU incorporation is rapidly becoming the gold standard method for DNA labeling.
CAS Number

61135-33-9
Structure Available

Yes
Salt Form

No
Molecular Formula

C₁₁H₁₂N₂O₅
Molecular Weight

252.22
Cell Permeable

Yes
Purity

≥98% by HPLC
Solubilities

DMSO (~10 mg/ml)
Handling

Protect from air and light
Tag Line

A DNA synthesis probe
Storage Condition

-20°C
Shipping Condition

gel pack
Shelf Life

36 months
MDL Number

MFCD01675687
PubChem CID

472172
PubChem CID Link
SMILES

C#CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)O
InChi

InChI=1S/C11H12N2O5/c1-2-6-4-13(11(17)12-10(6)16)9-3-7(15)8(5-14)18-9/h1,4,7-9,14-15H,3,5H2,(H,12,16,17)/t7-,8+,9+/m0/s1
InChi Key

CDEURGJCGCHYFH-DJLDLDEBSA-N
Data Sheet Link
MSDS Available

Yes