Anti-Nanog (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-0829R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  Nanog
• Specificity
  This antibody reacts specifically with Nanog
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Nucleus
• Antigen Source
  KLH conjugated synthetic peptide derived from mouse Nanog
• Gene ID
  71950
• Swiss Prot
  Q80Z64
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  ENK; ecat4; 2412E2Rik; Homeobox protein NANOG; ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog; Nanog
• Also known as
  Nanog Polyclonal Antibody
• Other name
  Anti-Nanog Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  Nanog  
• Gene symbol
  NANOG, NANOGP3, NANOGP8
• Short name
  Anti-Nanog
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  Nanog Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:20857
• Gene
  NANOG
• Long gene name
  Nanog homeobox
• Synonyms
  • FLJ12581
  • FLJ40451
• GenBank acession
  • AB093576
• Locus
  12p13.31
• Discovery year
  2003-09-10
• Entrez gene record
  79923
• Pubmed identfication
  • 12787505
  • 12787504
• RefSeq identity
  • NM_024865
• Classification
  • NKL subclass homeoboxes and pseudogenes
• VEGA ID
  OTTHUMG00000166019

Gene info

• Identity
  HGNC:23101
• Gene
  NANOGP3
• Long gene name
  Nanog homeobox pseudogene 3
• Locus
  6p12.1
• Discovery year
  2003-10-07
• Entrez gene record
  340217
• RefSeq identity
  • NG_004095
• Classification
  • NKL subclass homeoboxes and pseudogenes
• VEGA ID
  OTTHUMG00000184573

Gene info

• Identity
  HGNC:23106
• Gene
  NANOGP8
• Long gene name
  Nanog homeobox retrogene P8
• Synonyms gene name
  • Nanog homeobox pseudogene 8
• Locus
  15q14
• Discovery year
  2003-10-07
• Entrez gene record
  388112
• Pubmed identfication
  • 23173096
• RefSeq identity
  • NM_001355281
• Classification
  • NKL subclass homeoboxes and pseudogenes
• VEGA ID
  OTTHUMG00000166041

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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