-
Synonyms
Human Recombinant PRDX 4
-
Alternative_names
Peroxiredoxin 4, PRX-4, AOE37-2, PRDX4.
-
Description
An antioxidant protein implicated in a variety of cellular responses
-
Recombinant
Yes
-
Source
E. coli
-
Purity by SDS PAGE
≥90%
-
Assay
SDS-PAGE
-
Biological activity
Specific activity: approximately 230-310 pmole/min/µg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX 4 and peroxide for 20 min at room temperature.
-
Unit Definition
Specific activity is defined as the amount of hydroperoxide that 1 µg of enzyme can reduce at 25°C for 1 minute.
-
Molecular Weight
28.8 kDa
-
Storage Temp
-20°C
-
Shipping
gel pack
-
Shelf Life
12 months
-
Concentration
1 mg/ml
-
Appearance
Liquid
-
Physical form description
1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
-
Background Information
Peroxiredoxin-4 in humans is encoded by the PRDX4 gene. The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. It is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappa B.
-
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants