PHPT1, human recombinant

• Catalog number
  P1181-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10, 14 kDa phosphohistidine phosphatase
• Alternative_names
  bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10, 14 kDa phosphohistidine phosphatase
• Description
  Enzyme that exhibits phosphohistidine phosphatase activity
• Recombinant
  Yes
• Source
  E.coli
• Purity by SDS PAGE
  > 95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its ability to hydrolyze 1nmol of p-nitrophenyl phosphate per minute at pH7.5 at 37°C.
• Biological activity
  Specific activity is >120 units/mg
• Results
  >120 units/mg
• Molecular Weight
  15.9 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol
• Background Information
  PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCDCECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PHPT1  
• Gene symbol
  PHPT1
• Short name
  PHPT1, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  PHPT1, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:30033
• Gene
  PHPT1
• Long gene name
  phosphohistidine phosphatase 1
• Synonyms
  • PHP14
  • HSPC141
  • CGI-202
  • DKFZp564M173
  • bA216L13.10
• Synonyms name
  • phosphohistidine phosphatase 14kDa
  • sex-regulated protein janus-a
• GenBank acession
  • AF131857
• Locus
  9q34.3
• Discovery year
  2004-03-23
• Entrez gene record
  29085
• Pubmed identfication
  • 11042152
  • 8619474
• RefSeq identity
  • NM_014172
• Classification
  • Protein phosphatases
• VEGA ID
  OTTHUMG00000020950

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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