OxiSelect™ Aldehyde-Induced DNA Damage ELISA Combo test kit (Ethenoadenosine / Ethenocytidine quantification)
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Catalog number
STA-820-C
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Price
Please ask
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Size
96 assays
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We send this reagant on
Room temperature
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The temperatureerature for storage is
Upon receipt, store the Ethenoadenosine Standard, Ethenocytidine Standard, Anti-Ethenoadenosine Antibody and Anti-Ethenocytidine Antibody at -20ºC. Store all other components at 4ºC until their expiration dates.
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Technical file
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SDS or safty data sheet
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Type of reagent
Oxidative Stress/Damage
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Related techniques
Oxidative Stress/ Damage, DNA / RNA Damage and Repair, Aldehyde Induced DNA Damage Assays, Ethenoadenosine and Ethenocytidine ELISA Combo test kit
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Use of the reagent
The OxiSelect™ Aldehyde-Induced DNA Damage ELISA Combo test kit (Ethenoadenosine / Ethenocytidine quantification) is a competitive ELISA test kit allowing the quantification of ethenoadenosine or ethenocytidine adducts in DNA isolated from cells or tissues. Samples and standards are added to a provided microplate preabsorbed with etheno-damaged DNA. A primary antibody is added and binds competitively to ethenoadenosine in the unknown sample or to the etheno adduct in the DNA coated on the plate. After washing a secondary antibody is added. A low signal means most of the primary antibody was bound to the unknown, indicating a high level of etheno adducts in the sample. Each combo test kit can perform up to 96 assays combined for ethenoadenosine and ethenocytidine. The two targets are assayed in separate wells, but any well on the provided plate may be used for either target in any ratio (e.g. 64 wells for ethenoadenosine and 32 wells for ethenocytidine, or 48 wells for each).
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Cellular use
Detect as little as 50 nM of ethenoadenosine or 625 nM of ethenocytidine from DNA isolated from cells or tissues
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Protocol information
Ethenoadenosine and ethenocytidine are assayed in separate wells; each test kit can perform a combined total of 96 assays in any ratio of the two targets
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Reagent properties
Unknown samples are compared with a provided standard
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Living modified organisme in this reagent or LMO
Not applicable
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Species compatible to
Humans
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Use of this test kit or product
Genetics lab
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Antigen
a specific protein sequence
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,96
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Molecular weight
in kilo Danlto or kDa
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This reagent is purified
0.97
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Description
Induced protein genes, factors or kinases, increase the production of (an enzyme or other protein) at the level of genetic transcription.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
OxiSelect™ Aldehyde- DNA Damage ELISA Combo test kit (Ethenoadenosine / Ethenocytidine quantification)
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Technique
ELISA, dna, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Alternative name
OxiSelect™ Aldehyde-Induced Desoxyribonucleic acid Damage Enzyme-linked immunosorbent assay Combo test reagent (Ethenoadenosine / Ethenocytidine quantification)
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Alternative technique
elisas, tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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