Mouse LFA1a(Lymphocyte Function Associated Antigen 1 Alpha) ELISA Kit

• Catalog number
  ELK4957
• Price
  Please ask
• Size
  96T

• Alternative Names
  CD11a; ITG-AL; LFA-1; LFA1-A; ITGAL; Integrin,Alpha L; CD11 antigen-like family member A; Leukocyte function-associated molecule 1 alpha chain
• Assay Type
  Sandwich
• Sensitivity
  0.054ng/mL
• Standard
  10ng/mL
• Detection range
  0.156-10ng/mL
• Assay length
  3h
• Research Area
  Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
• Test principle
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Function Associated Antigen 1 Alpha (LFA1α). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Function Associated Antigen 1 Alpha (LFA1α). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Function Associated Antigen 1 Alpha (LFA1α), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Function Associated Antigen 1 Alpha (LFA1α) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Description
  The Mouse LFA1a(Lymphocyte Associated Antigen 1 Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood. Antigens are peptides or recombinant or native dependent on the production method.
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  LFA1a   Lymphocyte   Associated   Alpha  
• Gene symbol
  ITGAL
• Short name
  Mouse LFA1a(Lymphocyte Associated Antigen 1 Alpha) ELISA Kit
• Technique
  ELISA kit, antigen, ELISA, Mouse, antigenes, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
• Host
  mouse, antigen assay ELISA kit 1 Kit (96 Wells)
• Species
  Mouse, Mouses
• Alternative name
  Mouse LFA1a(Lymphocyte Function Associated protein 1 a) Enzyme-linked immunosorbent assay reagent
• Alternative technique
  antigenes, murine, elisas, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  lymphocyte

Gene info

• Identity
  HGNC:6148
• Gene
  ITGAL
• Long gene name
  integrin subunit alpha L
• Synonyms gene
  • CD11A
• Synonyms gene name
  • integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Synonyms
  • LFA-1
  • LFA1A
• Synonyms name
  • antigen CD11A (p180)
  • lymphocyte function-associated antigen 1, alpha polypeptide
• Locus
  16p11.2
• Discovery year
  1986-01-01
• Entrez gene record
  3683
• Pubmed identfication
  • 3284962
• Classification
  • Integrin alpha subunits
  • CD molecules
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000176964

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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