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Organism Species
Rattus norvegicus (Rat)
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Source
Polyclonal antibody preparation
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Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Item Name
Apoptosis Antagonizing Transcription Factor
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Immunogen
RPA440Ra01-Recombinant Apoptosis Antagonizing Transcription Factor (AATF)
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Image number
3
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Species reactivity
Rat
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Sequence of immunogen
AATF (Ser237~Gln523)
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Aplication
WB,IHC
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Clonality
Rabbit-polyclonal
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Concentration
500ug/ml
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Alternative Names
CHE1; DED; Rb-binding protein Che-1
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Applicable Secondary Antibody
SAA544Rb59, SAA544Rb58, SAA544Rb57, SAA544Rb18, SAA544Rb19
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Delivery condition
4℃ with ice bags
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Storage instructions
Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. This 1 is suited for programmed cell-death studies. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
AATF
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Short name
200ug-Anti-Apoptosis Antagonizing Transcription Factor (AATF)-polyclonal Antibody
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Technique
Polyclonal, apoptosis, Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, apoptosis pathway, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit
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Alternative name
200ug-Antibody toprogrammed cellular destruction Antagonizing Transcription Factor (AATF)-polyclonal (antibody to-)
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Alternative technique
polyclonals, celldeath, antibodies
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