MMP-13 Inhibitor Screening Kit (Fluorometric)

• Catalog number
  K2067-100
• Price
  Please ask
• Size
  100 assays

• Description
  For screening, studying and characterizing potential inhibitors of MMP-13
• Summary
  BioVision’s MMP-13 Inhibitor Screening Assay Kit provides a quick, easy and sensitive assay for screening, studying and characterizing potential inhibitors of MMP-13. In this assay, MMP-13 hydrolyzes a FRET-based MMP-13 substrate and releases unquenched by the catalysis of fluorescent group, Mca, which is detected fluorometrically at Ex/Em = 325/393 nm. A potent, specific MMP-13 inhibitor is also included in the kit.
• Detection Method
  Fluorescence (Ex/Em = 325/393 nm)
• Species Reactivity
  Mammalian
• Applications
  Screening or characterizing MMP-13 inhibitors.
• Sample Type
  Potential MMP-13 Inhibitors
• Features Benefits
  A potent, specific MMP-13 inhibitor is also included in the kit.,A quick, easy and sensitive assay for screening, studying and characterizing potential inhibitors of MMP-13.
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Matrix metalloproteinase-13 (MMP-13; Collagenase-3) is a zinc-dependent endopeptidase family member that is capable of degrading many protein components of the extracellular matrix (ECM) including type II collagen in articular cartilage and bone. It is produced and secreted as a 60-kDa inactive, precursor form (proMMP-13), which is activated to a 48-kDa form by extracellular proteinases such as plasmin, MMP-2, MMP-3 and MMP-14. MMP-13 has wide substrate specificity, as it degrades collagen types (I-IV) but preferentially hydrolyzes type II collagen. Additionally, it cleaves other ECM molecules including fibronectin, aggrecan, osteonectin etc. MMP-13 plays a major role in ECM remodeling during fetal bone development, post-natal bone remodeling, tissue remodeling etc. It is overexpressed in numerous pathological conditions including rheumatoid arthritis, osteoarthritis, atherosclerosis, aortic aneurysms, cancer etc. Thus, developing and screening of selective MMP-13 inhibitors is under intensive investigation for therapeutic intervention. BioVision’s MMP-13 Inhibitor Screening Assay Kit provides a quick, easy and sensitive assay for screening, studying and characterizing potential inhibitors of MMP-13. In this assay, MMP-13 hydrolyzes a FRET-based MMP-13 substrate and releases unquenched by the catalysis of fluorescent group, Mca, which is detected fluorometrically at Ex/Em = 325/393 nm. A potent, specific MMP-13 inhibitor is also included in the kit.
• Data Sheet Link
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  MMP-13   Inhibitor   Screening   Kit   Fluorometric  
• Gene symbol
  MMP13, MMP24, MMP14, PIRC58, MMP28, SNORD115-13, SNORD114-13, IGHV3OR16-13, IGLV3-13, MMP17
• Short name
  MMP-13 Inhibitor Screening Kit (Fluorometric)
• Alternative name
  MMP-13 suppressor detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:7159
• Gene
  MMP13
• Long gene name
  matrix metallopeptidase 13
• Synonyms gene name
  • matrix metalloproteinase 13 (collagenase 3)
• Synonyms
  • CLG3
• Synonyms name
  • collagenase 3
• GenBank acession
  • X75308
• Locus
  11q22.2
• Discovery year
  1994-11-20
• Entrez gene record
  4322
• Pubmed identfication
  • 8207000
• RefSeq identity
  • NM_002427
• Classification
  • M10 matrix metallopeptidases
  • Receptor ligands
• VEGA ID
  OTTHUMG00000165850

Gene info

• Identity
  HGNC:7172
• Gene
  MMP24
• Long gene name
  matrix metallopeptidase 24
• Synonyms gene name
  • matrix metalloproteinase 24 (membrane-inserted)
  • matrix metallopeptidase 24 (membrane-inserted)
• Synonyms
  • MT5-MMP
• Synonyms name
  • membrane-type 5 matrix metalloproteinase
• GenBank acession
  • AF131284
• Locus
  20q11.22
• Discovery year
  1999-08-06
• Entrez gene record
  10893
• Pubmed identfication
  • 10363975
• RefSeq identity
  • NM_006690
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000032330

Gene info

• Identity
  HGNC:7160
• Gene
  MMP14
• Long gene name
  matrix metallopeptidase 14
• Synonyms gene name
  • matrix metalloproteinase 14 (membrane-inserted)
  • matrix metallopeptidase 14 (membrane-inserted)
• Synonyms
  • MT1-MMP
• Synonyms name
  • membrane type 1 metalloprotease
  • membrane type 1-matrix metalloproteinase
• Locus
  14q11.2
• Discovery year
  1994-11-20
• Entrez gene record
  4323
• Pubmed identfication
  • 8015608
• RefSeq identity
  • NM_004995
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000028704

Gene info

• Identity
  HGNC:37562
• Gene
  PIRC58
• Long gene name
  piwi-interacting RNA cluster 58
• Locus
  13
• Discovery year
  2009-11-05
• Entrez gene record
  100313918
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:14366
• Gene
  MMP28
• Long gene name
  matrix metallopeptidase 28
• Synonyms gene name
  • matrix metalloproteinase 28
• Synonyms
  • MMP-25
  • MM28
  • EPILYSIN
  • MMP-28
• GenBank acession
  • AF315683
• Locus
  17q12
• Discovery year
  2001-01-09
• Entrez gene record
  79148
• Pubmed identfication
  • 11121398
  • 11255011
• RefSeq identity
  • NM_024302
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000188387

Gene info

• Identity
  HGNC:33032
• Gene
  SNORD115-13
• Long gene name
  small nucleolar RNA, C/D box 115-13
• Synonyms
  • HBII-52-13
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033450
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003305.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33001
• Gene
  SNORD114-13
• Long gene name
  small nucleolar RNA, C/D box 114-13
• Synonyms
  • 14q(II-13)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767591
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003206.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5637
• Gene
  IGHV3OR16-13
• Long gene name
  immunoglobulin heavy variable 3/OR16-13 (non-functional)
• Synonyms gene name
  • immunoglobulin heavy variable 3/OR16-13
• Synonyms
  • IGHV3/OR16-13
• GenBank acession
  • Z29610
• Locus
  16p11.2
• Discovery year
  2000-04-17
• Entrez gene record
  100287372
• RefSeq identity
  • NG_011771
• Classification
  • Immunoglobulin heavy (IGH) orphons
• VEGA ID
  OTTHUMG00000176357

Gene info

• Identity
  HGNC:5899
• Gene
  IGLV3-13
• Long gene name
  immunoglobulin lambda variable 3-13 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable 3-13
  • immunoglobulin lambda variable 3-13 pseudogene
• GenBank acession
  • X97463
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28801
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151215

Gene info

• Identity
  HGNC:7163
• Gene
  MMP17
• Long gene name
  matrix metallopeptidase 17
• Synonyms gene name
  • matrix metalloproteinase 17 (membrane-inserted)
  • matrix metallopeptidase 17 (membrane-inserted)
• Synonyms
  • MT4-MMP
• GenBank acession
  • X89576
• Locus
  12q24.33
• Discovery year
  1996-11-13
• Entrez gene record
  4326
• Pubmed identfication
  • 9878265
• RefSeq identity
  • NM_016155
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000168050

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