Vascular Endothelial Growth Factor (VEGF) (Western Blot Control)

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Test

Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
Description

Isotype or positive controls by peptides, antibodies and deactivated samples. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

Gene target

Vascular Endothelial Growth Factor VEGF
Gene symbol

VEGFA, VEGFC, VEGFD
Short name

Vascular Endothelial Growth Factor (VEGF) ( Blot Control)
Technique

Western Blot, Control, Blot, western Blots, controls
Alternative name

Vascular Endothelial Growth Factor (VEGF) (Western Blot reference)
Alternative technique

controls, western
Tissue

control, vascular

Identity

HGNC:12680
Gene

VEGFA
Long gene name

vascular endothelial growth factor A
Synonyms gene
- VEGF
Synonyms gene name
- vascular endothelial growth factor
Synonyms
- VEGF-A
- VPF
GenBank acession
- AB021221
Locus

6p21.1
Discovery year

1994-01-10
Entrez gene record

7422
Pubmed identfication
- 8786112
RefSeq identity
- NM_001025366
Classification
- VEGF family
VEGA ID

OTTHUMG00000014745
Locus Specific Databases
- ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

Identity

HGNC:3708
Gene

VEGFD
Long gene name

vascular endothelial growth factor D
Synonyms gene
- FIGF
Synonyms gene name
- c-fos induced growth factor (vascular endothelial growth factor D)
Synonyms
- VEGF-D
GenBank acession
- AJ000185
Locus

Xp22.2
Discovery year

1997-03-27
Entrez gene record

2277
Pubmed identfication
- 9479493
RefSeq identity
- NM_004469
Classification
- VEGF family
VEGA ID

OTTHUMG00000021175
Locus Specific Databases
- Global Variome shared LOVD

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data