Anti-REF1/APEX1 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-1406R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  REF1/APEX1
• Specificity
  This antibody reacts specifically with REF1/APEX1
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human APEX1
• Gene ID
  328
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq].
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  mitochondrial; AP endonuclease 1; Ape1; AP endonuclease class I; AP lyase; APE 1; APE; APE-1; APEN; APEX 1; APEX; APEX nuclease multunctional DNA repair enzyme 1; Apex nuclease 1; APEX nuclease; APEX1; APEX1_HUMAN; Apurinic endonuclease; Apurinic-apyrimidinic endonuclease 1; Apurinic/apyrimidinic abasic endonuclease; Apurinic/apyrimidinic endonuclease 1; Apurinic/apyrimidinic exonuclease; APX; BAP1; Deoxyribonuclease apurinic or apyrimidinic; DNA apurinic or apyrimidinic site lyase; DNA-apurinic or apyrimidinic site lyase; DNA-apurinic or apyrimidinic site lyase, mitochondrial; EC 4.2.99.18; HAP 1; HAP1; Human Apurinic endonuclease 1; Apurinic/Apyrimidic eEndonuclease 1; MGC139790; Multunctional DNA repair enzyme; Redox effector factor 1; Redox factor 1; Redox factor-1; REF 1; REF 1 protein; REF-1; REF1; REF1 protein.
• Also known as
  REF1/APEX1 Polyclonal Antibody
• Other name
  Anti-REF1/APEX1 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  REF1/APEX1  
• Gene symbol
  APEX1, TFPI2
• Short name
  Anti-REF1/APEX1
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  REF1/APEX1 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:587
• Gene
  APEX1
• Long gene name
  apurinic/apyrimidinic endodeoxyribonuclease 1
• Synonyms gene
  • APEX
• Synonyms gene name
  • APEX nuclease (multifunctional DNA repair enzyme)
  • APEX nuclease (multifunctional DNA repair enzyme) 1
• Synonyms
  • APE
  • REF1
  • HAP1
  • APX
  • APEN
  • REF-1
  • APE-1
• GenBank acession
  • X59764
• Locus
  14q11.2
• Discovery year
  1997-05-22
• Entrez gene record
  328
• RefSeq identity
  • NM_001641
• Classification
  • SET complex
  • Endoribonucleases
• VEGA ID
  OTTHUMG00000029544
• Locus Specific Databases
  • ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

Gene info

• Identity
  HGNC:11761
• Gene
  TFPI2
• Long gene name
  tissue factor pathway inhibitor 2
• Synonyms
  • PP5
  • TFPI-2
  • REF1
• GenBank acession
  • L27624
• Locus
  7q21.3
• Discovery year
  1999-07-14
• Entrez gene record
  7980
• Pubmed identfication
  • 7896752
  • 8945635
• RefSeq identity
  • NM_006528
• VEGA ID
  OTTHUMG00000022963
• Locus Specific Databases
  • LRG_647

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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