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Available ordering format
Liquid in 0.9% NaCl, 0.1% sodium azide
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Immunogen
whole, formaldehyde killed, bacteria, strains PAO1 and Habs1
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Raised in
Hen
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgY fraction
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
Western blot (WB)
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Connected products
Secondary antibodies
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Recommended dilutions for use
1: 1000 - 1: 5 000 with standard ECL (WB), 1: 1000 - 1: 5 000 (ELISA)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Pseudomonas aeruginosa strains PAO1 and Habs1
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Possible reactivity
n. a.
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
reacts with P. aeruginosa strains PAO1 and Habs1; it will also crossreact with other P. aeruginosa strains in ELISA and immunoblotting
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References
to be added when available
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Scientific context
Pseudomonas aeruginosa is Gram-negative, aerobic, rod-shaped bacteria with unipolar motility. An opportunistic human pathogen, P. aeruginosa is also an opportunistic pathogen of plants. P. aeruginosa bacterias are clinically important because they are resistant to most antibiotics and they are capable of surviving in conditions that few other organisms can tolerate. Pseudomonas is often encountered in hospital and clinical work because it is a major cause of hospital acquired (nosocomal) infections. Its main targets are immunocompromised individuals, burn victims, and individuals on respirators or with precipitation procedure followed by indwelling catheters. Additionally, these pathogens colonize the lungs of cystic fibrosis patients. P. aeruginosa is often identified by its pearlescent appearance and grape-like odor in vitro. Definitive clinical identification of P. aeruginosa includes identifying the production of both pyocyanin and fluorescein as well as its ability to grow at 42°C.
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Notes
the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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About
Pseudomonas is a Gram-negative bacteria. Pseudomonas genomic sequences are used in medical research. A problem in hostpital is often the Pseudomonas Aeruginosa containing multi drug resistancy properties that are transmitted through plasmids. The MDR property is transmitter by the R plasmid.
