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Immunogen
Synthetic peptide from the N-terminal to mid of Human SQSTM1 (aa. 195-205)
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Antibody s target
Human SQSTM1
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Antibody s full description
Rabbit Anti-Human SQSTM1 Polyclonal
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Primary research fields
Cancer, Autophagy, Cell Signaling, Trafficking, Epigenetics, Cardiovascular System, Heart
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
EBI3-associated protein of 60 kDa Antibody, ORCA Antibody, Osi Antibody, SQSTM 1 Antibody, ZIP 3 Antibody, A170 Antibody, SQSTM1 Antibody, Ubiquitin binding protein p62 Antibody, Protein kinase C-zeta-interacting protein Antibody, FTDALS3 Antibody, Ubiquitin-binding protein p62 Antibody, PKC-zeta-interacting protein Antibody, EBIAP Antibody, Sqstm1 Antibody, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa Antibody, EBI3 associated protein p60 Antibody, EBI3 associated protein of 60 kDa Antibody, SQSTM_HUMAN Antibody, OSF-6 Antibody, Paget disease of bone 3 Antibody, p62B Antibody, p60 Antibody, PDB 3 Antibody, EBI 3 associated protein p60 Antibody, MGC127197 Antibody, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Antibody, EBI 3 associated protein of 60 kDa Antibody, ZIP3 Antibody, STAP Antibody, p62 Antibody, Sequestosome 1 Antibody, OSIL Antibody, STONE14 Antibody, Phosphotyrosine independent ligand for the Lck SH2 domain p62 Antibody, PDB3 Antibody, Oxidative stress induced like Antibody, Sequestosome-1 Antibody, ZIP Antibody,
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Verified applications
WB, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~60 kDa. Band at 40 kDa is isoform 2.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-636 was sufficient for detection of SQSTM1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Late Endosome, Lysosome, Cytoplasmic Vesicle, Autophagosome, Nucleus, Endoplasmic Reticulum, Cytoplasm, P-Body
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Tissue specificity
Ubiquitously expressed.
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Scientific context
SQSTM1 or Sequestome 1 (p62) is a ubiquitin binding protein that interacts with ubiqutin, providing a scaffold for several signaling proteins and triggering degradation of proteins throught the proteasome or lysossome. This gene regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Mutations in this gene result in sporadic and familial Paget disease of bone. SQSTM1 also binds autophagosomal membrane protein LC3/ATG8.
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Bibliography
1. Kirkin V., et al. (2009) Mol Cell. 34: 259-269. 2. Seibenhener M.L., et al. (2007) FEBS Lett. 581: 175-179. 3. Komatsu M., et al. (2010) Nat Cell Biol. 12: 213-223. 4. Bjørkøy G., et al. (2006) Autophagy. 2: 138-139. 5. Pankiv S., et al. (2007) J Biol Chem. 282: 24131-24145.
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Released date
29/Aug/2016
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NCBI number
NP_001135770.1
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Gene number
8878
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Protein number
Q13501
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-636). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-636) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Late Endosome, Lysosome, Cytoplasmic Vesicle, Autophagosome, Nucleus, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SQSTM1 Antibody (D) Composite. | Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 47.7 kDa SQSTM1 protein using Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-636). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-636) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 47.7 kDa. Other Band(s): ~65 kDa in all lysates. Rabbit Anti-SQSTM1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on NIH 3T3 (Mouse Fibroblast cell line) (SPC-636) | Rabbit Anti-SQSTM1 Antibody used in Western blot (WB) on HeLa and 293Trap cell lysates (SPC-636)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
RPE, SQSTM1
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Short name
Rabbit Anti- SQSTM1 Polyclonal: RPE
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
RPE
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman sequestosome 1 polyclonal: RPE
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
sequestosome 1, A170 and OSIL and p60 and p62 and p62B and PDB3 and ZIP3, SQSTM1 and IDBG-61811 and ENSG00000161011 and 8878, K63-linked polyubiquitin binding, nuclei, Sqstm1 and IDBG-168358 and ENSMUSG00000015837 and 18412, BT.105440 and IDBG-640935 and ENSBTAG00000015591 and 338045
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