Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1
Antibody's other name
ACCN2 Antibody, Acid-sensing (proton-gated) ion channel Antibody, Acid-sensing ion channel 1 Antibody, Amiloride-sensitive cation channel 2 neuronal Antibody, BNAC2 Antibody, Brain sodium channel 2 Antibody
Primary research fields
Neuroscience, Ion Channels, Sodium Channels, Proton-Gated Sodium Channels
WB, IHC, ICC/IF
WB (1:1000); optimal dilutions for assays should be determined by the user.
Protein G Purified
Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Blue Ice or 4ºC
Antibody certificate of analysis
1 µg/ml of SMC-427 was sufficient for detection of ASIC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody in cell
See included datasheet or contact our support service
Acid sensing ion channel ASIC1, also designated ACCN2, BNAC2 and ASIC1a, is present in brain as a 4.3-kb transcript with localization to rat dorsal root ganglia. Insitu hybridization of rat brain suggests that ASIC1 is most abundant in the main olfactory bulb, cerebral cortex, hippocampal formation, habenula, basolateral amygdaloid nuclei and cerebellum. ASIC1 and H+-gated currents may contribute to the development of fear and anxiety.
1. Garcia-Anoveros J., Derfler B., Neville-Golden J., Hyman B.T. and Corey D.P. (1997) Proc. Natl. Acad. Sci. USA 94: 1459-1464. 2. Waldmann R., Champigny G., Bassilana F., Heurteaux C. and Lazdunski M. (1997) Nature. 386: 173-177. 3. Grunder S., Geisler H.S., Rainier S. and Fink J.K. (2001) Eur. J. Hum. Genet. 9:672-676. 4. Chen C.C., Zimmer A., Sun W.H., Hall J., Brownstein M.J. and Zimmer A. (2002) Proc. Natl. Acad. Sci. USA 99: 8992-8997. 5. Wemmie J.A., et al. (2004) Proc. Natl. Acad. Sci. USA 101: 3621-3626 .
To be tested
To be tested
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ASIC1 Monoclonal Antibody, Clone S271-44 (SMC-427). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ASIC1 Monoclonal Antibody (SMC-427) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ASIC1 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of ASIC1 protein using Mouse Anti-ASIC1 Monoclonal Antibody, Clone S271-44 (SMC-427). Primary Antibody: Mouse Anti-ASIC1 Monoclonal Antibody (SMC-427) at 1:100, 1:250, 1:500, and 1:1000. Mouse Anti-ASIC1 Antibody [S271-44] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-427) Mouse Anti-ASIC1 Antibody [S271-44] used in Western Blot (WB) on Rat brain lysates (SMC-427)
Country of production
Total weight (kg)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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