PFKFB1/4 Colorimetric Cell-Based ELISA
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Catalog number
CB6043
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Price
Please ask
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Size
One 96-Well Plate
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Description
PFKFB1/4 Colorimetric Cell-Based ELISA is an appropriate, lysatefree, high throughput and high sensitivity assay kit that can check PFKFB1/4protein and its expression profile in cells. This kit can be used in order to measur with a hight precision the relative amounts of PFKFB1/4insid the cultured cells as well as detection of the effects that various inhibitors or treatments (ie. siRNA or chemicals), or activators have on PFKFB1/4.
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Target Name
PFKFB1/4
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Assay Type
Non-Phospho Cell-Based ELISA
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Detection Method
Colorimetric 450 nm
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Synonyms
6PF-2-K/Fru- 2,6-P2ASE liver isozyme; F261; F6PK; PFRX ; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1.
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NCBI Gene Symbol
PFKFB1
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Storage
4°C
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Stability
140 Months
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Dynamic Range
> 5000 Cells
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Recommended use
Research use only
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Contact us
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Technical Service
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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Additional description
For cells, cell lines and tissues in culture till half confluency.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction. E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
PFKFB1
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Short name
PFKFB1/4 Colorimetric -Based ELISA
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Technique
colorimetric, ELISA, colorimetry, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Alternative name
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1/4 colorimetry cellular-Based Enzyme-linked immunosorbent assay
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Alternative technique
assays, elisas
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Alternative to gene target
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1, F6PK and HL2K and PFRX, PFKFB1 and IDBG-71927 and ENSG00000158571 and 5207, fructose-2, Cytoplasm, Pfkfb1 and IDBG-178179 and ENSMUSG00000025271 and 18639, PFKFB1 and IDBG-637557 and ENSBTAG00000000172 and 282304
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Tissue
cell
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Gene info
MeSH Data
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Name
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Concept
Scope note:
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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