Recombinant Human CCNA1 / Cyclin-A1 Protein (His tag)

• Catalog number
  PKSH031307-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  CT146
• Activity
  Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK1. The EC50 of biotinylated human CDK1 is 0.02-0.04 μg/ml.Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK2-His. The EC50 of biotinylated human CDK2-His is 0.07-0.15 μg/ml.
• Sequence
  Met 1-Gln 465
• Fusion tag
  N-His
• Accession
  NP_003905.1
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞96 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  54.6 kDa
• AP Mol Mass
  50 kDa
• Formulation
  Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
• Background
  Cyclin A1 is a member of the highly conserved cyclin family that is characterized by a dramatic periodicity in protein abundance, and belongs to the A-type cyclin subfamily. The mammalian A-type cyclin family consists of two members: cyclin A1 and cyclin A2. Different cyclins exhibit distinct expression. Cyclin A1 is expressed in mice exclusively in the germ cell lineage and high rate of cyclinA1 is found in human testis and certain myeloid leukaemia cells. Cyclin A1 is primarily function in the control of meiosis. It serves as regulator subunits binding to cyclin-dependent kinase 1 (Cdk1) and cyclin-dependent kinase 2 (Cdk2), which give two different kinase activities, one appearing in S phase, the other in G2. Through this, cyclin A1 operate the entry and progression in cell cycle. High frequency of cyclin A1 overexpression has been observed in acute myelocytic leukemias, especially those that are at the promyelocyte and myeloblast stages of development.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CCNA1   Cyclin-A1   Protein   His   tag  
• Gene symbol
  RFC4, RFC2, SNAR-A1, PCDHGA1, ZNF2, SCAF1, FALEC, HNRNPA1, RFC1, EDA
• Short name
  Recombinant CCNA1 / Cyclin-A1 Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens CCNA1 / Cyclin-A1 Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:9972
• Gene
  RFC4
• Long gene name
  replication factor C subunit 4
• Synonyms gene name
  • replication factor C (activator 1) 4 (37kD)
  • replication factor C (activator 1) 4, 37kDa
• Synonyms
  • A1
  • RFC37
• Synonyms name
  • A1 37 kDa subunit
  • activator 1 37 kDa subunit
  • RFC 37 kDa subunit
• Locus
  3q27.3
• Discovery year
  1994-10-14
• Entrez gene record
  5984
• Pubmed identfication
  • 7774928
• RefSeq identity
  • NM_002916
• Classification
  • AAA ATPases
• VEGA ID
  OTTHUMG00000156515

Gene info

• Identity
  HGNC:9970
• Gene
  RFC2
• Long gene name
  replication factor C subunit 2
• Synonyms gene name
  • replication factor C (activator 1) 2 (40kD)
  • replication factor C (activator 1) 2, 40kDa
• Synonyms
  • A1
  • RFC40
• Synonyms name
  • activator 1
• Locus
  7q11.23
• Discovery year
  1994-10-14
• Entrez gene record
  5982
• Pubmed identfication
  • 1313560
  • 7774928
• RefSeq identity
  • NM_181471
• Classification
  • AAA ATPases
• VEGA ID
  OTTHUMG00000023239

Gene info

• Identity
  HGNC:34304
• Gene
  SNAR-A1
• Long gene name
  small NF90 (ILF3) associated RNA A1
• Synonyms gene name
  • small nuclear ILF3/NF90-associated RNA A1
  • small ILF3/NF90-associated RNA A1
• Synonyms
  • SNAR-A53113498
• GenBank acession
  • EU071051
• Locus
  19q13.33
• Discovery year
  2008-06-20
• Entrez gene record
  100126798
• Pubmed identfication
  • 17855395
• RefSeq identity
  • NR_004435
• Classification
  • Small NF90 (ILF3) associated RNAs

Gene info

• Identity
  HGNC:8696
• Gene
  PCDHGA1
• Long gene name
  protocadherin gamma subfamily A, 1
• Synonyms
  • PCDH-GAMMA-A1
• GenBank acession
  • AF152318
• Locus
  5q31
• Discovery year
  2000-06-28
• Entrez gene record
  56114
• Pubmed identfication
  • 10380929
• RefSeq identity
  • NM_018912
• Classification
  • Clustered protocadherins
• VEGA ID
  OTTHUMG00000163678

Gene info

• Identity
  HGNC:12991
• Gene
  ZNF2
• Long gene name
  zinc finger protein 2
• Synonyms gene name
  • zinc finger protein 2 (A1-5)
• Synonyms
  • A1-5
  • ZNF661
  • Zfp661
• GenBank acession
  • X60152
• Locus
  2q11.1
• Discovery year
  1989-05-31
• Entrez gene record
  7549
• Pubmed identfication
  • 8183940
  • 1945843
• RefSeq identity
  • NM_021088
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000155150

Gene info

• Identity
  HGNC:30403
• Gene
  SCAF1
• Long gene name
  SR-related CTD associated factor 1
• Synonyms
  • SR-A1
  • FLJ00034
• GenBank acession
  • AK024444
• Locus
  19q13.3-q13.4
• Discovery year
  2007-08-09
• Entrez gene record
  58506
• Pubmed identfication
  • 11461075
• RefSeq identity
  • NM_021228
• VEGA ID
  OTTHUMG00000183238

Gene info

• Identity
  HGNC:43713
• Gene
  FALEC
• Long gene name
  focally amplified long non-coding RNA in epithelial cancer
• Synonyms gene
  • LINC00568
• Synonyms gene name
  • long intergenic non-protein coding RNA 568
• Synonyms
  • ncRNA-a1
  • FAL1
• Synonyms name
  • focally amplified lncRNA on chromosome 1
• GenBank acession
  • AL713297
• Locus
  1q21.2
• Discovery year
  2012-02-02
• Entrez gene record
  100874054
• Pubmed identfication
  • 20887892
  • 25203321
  • 29196104
• RefSeq identity
  • NR_051960
• Classification
  • Long non-coding RNAs with non-systematic symbols
• VEGA ID
  OTTHUMG00000035849

Gene info

• Identity
  HGNC:5031
• Gene
  HNRNPA1
• Long gene name
  heterogeneous nuclear ribonucleoprotein A1
• Synonyms gene
  • HNRPA1
• Synonyms
  • hnRNP-A1
  • ALS20
• GenBank acession
  • BC009600
• Locus
  12q13.13
• Discovery year
  1991-05-21
• Entrez gene record
  3178
• Pubmed identfication
  • 1733858
  • 23455423
• RefSeq identity
  • NM_031157
• Classification
  • RNA binding motif containing
  • Spliceosomal A complex
  • Heterogeneous nuclear ribonucleoproteins
• VEGA ID
  OTTHUMG00000169702

Gene info

• Identity
  HGNC:9969
• Gene
  RFC1
• Long gene name
  replication factor C subunit 1
• Synonyms gene name
  • replication factor C (activator 1) 1 (145kD)
  • replication factor C (activator 1) 1, 145kDa
• Synonyms
  • A1
  • PO-GA
  • RFC140
  • MHCBFB
• GenBank acession
  • L23320
• Locus
  4p14
• Discovery year
  1994-10-14
• Entrez gene record
  5981
• Pubmed identfication
  • 8114700
• RefSeq identity
  • NM_002913
• Classification
  • AAA ATPases
• VEGA ID
  OTTHUMG00000099363

Gene info

• Identity
  HGNC:3157
• Gene
  EDA
• Long gene name
  ectodysplasin A
• Synonyms gene
  • ED1
  • EDA2
  • ODT1
• Synonyms gene name
  • ectodermal dysplasia 1, anhidrotic
  • oligodontia 1
• Synonyms
  • EDA1
  • XLHED
  • HED
  • XHED
  • ED1-A1
  • ED1-A2
  • EDA-A1
  • EDA-A2
• GenBank acession
  • U59227
• Locus
  Xq13.1
• Discovery year
  2001-06-22
• Entrez gene record
  1896
• Pubmed identfication
  • 8696334
  • 18657636
  • 16583127
• RefSeq identity
  • NM_001399
• Classification
  • MicroRNA protein coding host genes
  • Tumor necrosis factor superfamily
• VEGA ID
  OTTHUMG00000021764
• Locus Specific Databases
  • Global Variome shared LOVD

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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