pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM plasmid]
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Catalog number
LL210PA-1
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Price
Please ask
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Size
10 ug
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Shipping Temperature
Blue Ice/ Dry Ice
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Description
Lenti kit or vector with CMV or EF promotors for producing Lentivirus (lenti-, Latin for "slow") or lentiviral particles in 293t cells. Lentiviruses are characterized by a long incubation period and often Puro selected. Lentiviruses can deliver a significant amount of viral RNA into the DNA of the host cell and have the unique ability among retroviruses of being able to infect non-dividing cells. They are one of the most efficient methods of a gene delivery vector. The are packaged in HEK 293T cells to get the highest possible lentiviral titers of 108 IFU/ml. The Luc reporter is the expression of the firefly luciferase gene. Puro stands for puromicin selection.
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Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Gene symbol
TRIM27
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Short name
pLL-EF1a-RFP-T2A-Puro [ -LabelerTM plasmid]
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Technique
plasmid, lenti, Lentivectors and viral particles, plasmids in 1
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Label
puro
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Alternative name
pLL-EF1a-Red Fluorescent-T2A-Puro [lentiviral-LabelerTM plasmid]
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Alternative technique
plasmids
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Virus
lenti
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Use of written, printed, or graphic materials upon or accompanying a drug container or wrapper. It includes contents, indications, effects, dosages, routes, methods, frequency and duration of administration, warnings, hazards, contraindications, side effects, precautions, and other relevant information.
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Tree numbers
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Qualifiers
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