Human Recombinant HSC70 Protein
#
-
Catalog numberSPR-106C
-
Price:
-
Size2x100 µg
-
-
Stock availabilityIn Stock
-
Scientific contextHSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
-
Protein targetHSC70
-
Protein reactivityHuman
-
Certificate of analysisThis product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.2µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
-
Protein descriptionActive Human Recombinant HSC70 Protein
-
Other nameConstitutive heat shock protein 70 Protein, HSC54 Protein, HSC71 Protein, HSC73 Protein, HSP71 Protein, HSP74 Protein, HSPA10 Protein, HSPA8 Protein, LAP1 Protein, NIP71 Protein
-
Primary research areaCancer, Heat Shock
-
CategoryProtein
-
Brand namenone
-
OriginRecombinant
-
NCBI numberAF352832
-
Gene number3312
-
Protein numberP11142
-
Verified applicationsWB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA
-
Relevant bio activityATPase active
-
Protein expression modelE. coli
-
Protein charastericsSee included datasheet.
-
Peptide sequenceSee included datasheet.
-
Protein purificationAffinity Purified
-
Purity pourcentage>90% High purity
-
Recommended buffer for storage20mM Tris/HCl pH7.5, 1mM EDTA
-
Protein concentrationLot/batch specific. See included datasheet.
-
Protein specificity~70 kDa
-
Protein tagHis tag
-
Storage recommendations-20°C
-
Shipping recommendationsBlue Ice or 4°C
-
Supplementary useful informationPlease see included datasheet or contact us
-
Protein cell localizationCytoplasm, Melanosome
-
Bibliography1. Brown C. L., et al. (1993) J.Cell Biol. 120(5): 1101-1112 2. Boorstein W. R., Ziegelhoffer T. & Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell. 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 6. Brown C. L., et al. (1996) J. Biol. Chem. 271(2): 833-840.
-
Release date1-Sep-2007
-
PubMed number28026090, 26244546, 25939977, 25979775, 23291500, 20084527, 19209902
-
Tested applicationsWestern Blot, Functional Assay, Intrinsic fluorescence spectroscopy, Western Blot Control, Functional Assay, Functional Assay, Western Blot Control
-
Tested species reactivityHuman, Mouse, Photinus pyralis
-
Representative figure legendSDS-PAGE of 73kDa Hsc70 protein (SPR-106). SDS-Page of human HSC70 Protein (SPR-106)
-
WarningsNon-hazardous materials
-
Protein originCanada
-
Total weight kg1.4
-
Net weight g0.2
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
SourceRecombinants or rec. proteins
-
Grouprecombinants
-
Gene target
-
Gene symbolHSPA8
-
Short nameRecombinant HSC70 Protein
-
TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
SpeciesHuman, Humans
-
Alternative nameH. sapiens Rec. HSC70 Protein
-
Alternative techniquerec
-
Gene info
-
Identity
-
Gene
-
Long gene nameheat shock protein family A (Hsp70) member 8
-
Synonyms gene
-
Synonyms gene name
- heat shock 70kD protein 8
- heat shock 70kDa protein 8
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1995-05-08
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Spliceosomal P complex
- Heat shock 70kDa proteins
- Spliceosomal C complex
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data