Rabbit Anti-P. falciparum HSP60 (P. falciparum) Polyclonal: RPE

  • Catalog number
    SPC-185D-RPE
  • Price
    Please ask
  • Size
    100 µg
  • Immunogen
    Recombinant full length PfHSP60
  • Antibody s target
    P. falciparum HSP60
  • Antibody s full description
    Rabbit Anti-P. falciparum HSP60 (P. falciparum) Polyclonal
  • Primary research fields
    Cancer, Heat Shock
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    CH60_PLAFG Antibody, Chaperonin CPN60 Antibody, mitochondrial Antibody
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Antibody s reactivity
    Plasmodium falciparum, Bacteria, E. coli (Escherichia coli)
  • Antibody s recommended dilutions for use
    WB (1:2000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1.83 mg/ml
  • Antibody s specificity
    Detects ~ 60kDa. Cross-reacts with E.coli HSP60, GroEl.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    0.9 µg/ml of SPC-185 was sufficient for detection of PfHSP60 in 20 µg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Mitochondrion, Mitochondrion Matrix
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
  • Bibliography
    1. Hartl F.U. (1996) Nature 381: 571-579. 2. Bukau B., and Horwich A.L. (1998) Cell 92: 351-366. 3. Hartl F.U and Hayer-Hartl M. (2002) Science 295: 1852-1858. 4. Jindal S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283. 5. La Verda D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh H., et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7. Gupta S. and Knowlton A.A. J. Cell Mol Med. 9: 51-58. 8. Deocaris C.C., et al. (2006) Cell Stress Chaperones 11: 116-128. 9. Lai H.C., et al. (2007) Am. J. Physiol. Endocrinol. Metab 292: E292-E297.
  • Released date
    27/Apr/2009
  • NCBI number
    XM_001347402.1
  • Gene number
    Refer to GenBank
  • Protein number
    P34940
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure legend
    Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (SPC-185). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (SPC-185) at 1:1000. Rabbit Anti-HSP60 Antibody used in Western blot (WB) on Parasite Lysates (SPC-185)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Gene symbol
    RPE, HSPD1
  • Short name
    Rabbit Anti-P. falciparum HSP60 (P. falciparum) Polyclonal: RPE
  • Technique
    Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    RPE
  • Alternative name
    production species: rabbit Antibody toP. falciparum HSP60 (P. falciparum) polyclonal: RPE
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    heat shock protein family D (Hsp60) member 1
  • Synonyms gene
  • Synonyms gene name
    • heat shock 60kD protein 1 (chaperonin)
    • spastic paraplegia 13 (autosomal dominant)
    • heat shock 60kDa protein 1 (chaperonin)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1991-07-19
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Chaperonins
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
Filters
Contact
Chat with gentaur.com employee