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Rabbit Anti-P. falciparum HSP60 (P. falciparum) Polyclonal: RPE

  • Catalog number
    SPC-185D-RPE
  • Product name
    Rabbit Anti-P. falciparum HSP60 (P. falciparum) Polyclonal: RPE
  • Size
    100 µg
  • Label
    RPE
  • Immunogen
    Recombinant full length PfHSP60
  • Antibody s target
    P. falciparum HSP60
  • Antibody s full description
    Rabbit Anti-P. falciparum HSP60 (P. falciparum) Polyclonal
  • Primary research fields
    Cancer, Heat Shock
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    CH60_PLAFG Antibody, Chaperonin CPN60 Antibody, mitochondrial Antibody
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Plasmodium falciparum, Bacteria, E. coli (Escherichia coli)
  • Antibody s recommended dilutions for use
    WB (1:2000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1.83 mg/ml
  • Antibody s specificity
    Detects ~ 60kDa. Cross-reacts with E.coli HSP60, GroEl.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    0.9 µg/ml of SPC-185 was sufficient for detection of PfHSP60 in 20 µg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Mitochondrion, Mitochondrion Matrix
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
  • Bibliography
    1. Hartl F.U. (1996) Nature 381: 571-579. 2. Bukau B., and Horwich A.L. (1998) Cell 92: 351-366. 3. Hartl F.U and Hayer-Hartl M. (2002) Science 295: 1852-1858. 4. Jindal S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283. 5. La Verda D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh H., et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7. Gupta S. and Knowlton A.A. J. Cell Mol Med. 9: 51-58. 8. Deocaris C.C., et al. (2006) Cell Stress Chaperones 11: 116-128. 9. Lai H.C., et al. (2007) Am. J. Physiol. Endocrinol. Metab 292: E292-E297.
  • Released date
    27/Apr/2009
  • NCBI number
    XM_001347402.1
  • Gene number
    Refer to GenBank
  • Protein number
    P34940
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (SPC-185). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (SPC-185) at 1:1000. Rabbit Anti-HSP60 Antibody used in Western blot (WB) on Parasite Lysates (SPC-185)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    P falciparum HSP60 P falciparum RPE
    • Gene info
    Gene info
  • Gene symbol
    RPE, HSPD1
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti-P. falciparum HSP60 (P. falciparum) Polyclonal: RPE
  • label filter
    • RPE
  • technique filter
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies