gentaur logogentaur logo
x

Rabbit Anti-Human ULK3 Polyclonal: ATTO 700

  • Catalog number
    SPC-642D-A700
  • Product name
    Rabbit Anti-Human ULK3 Polyclonal: ATTO 700
  • Size
    100 µg
  • Label
    ATTO 700
  • Immunogen
    Synthetic peptide from the N-terminal of Human ULK3 (aa. 166-177)
  • Antibody s target
    Human ULK3
  • Antibody s full description
    Rabbit Anti-Human ULK3 Polyclonal
  • Primary research fields
    Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Serine/threonine-protein kinase ULK3 Antibody, EC:2.7.11.1 Antibody, Ulk3 Antibody, DKFZP434C131 Antibody, Unc-51-like kinase 3 Antibody, FLJ90566 Antibody, ULK3_HUMAN Antibody, unc 51 like kinase 3 (C. elegans) Antibody, ULK3 Antibody,
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~53 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-642 was sufficient for detection of ULK3 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord.
  • Scientific context
    UNC-51 like kinase 3, ULK3, is a serine-threonine protein kinase that acts as a regulator of SHH signaling and autophagy. Is phosphorylates in vitro GLI2.
  • Bibliography
    1. Malovergan A., et al. (2010) J Biol Chem. 285(39): 30079-30090. 2. Young A.R., et al. (2009) Genes Dev. 23(7):798-803.
  • Released date
    29/Aug/2016
  • NCBI number
    NP_001092906.3
  • Gene number
    25989
  • Protein number
    Q6PHR2
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ULK3 Antibody (D) Composite. | Western blot analysis of Human A549 cell lysates showing detection of 53.4 kDa ULK3 protein using Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 53.4 kDa. Other Band(s): ~25 kDa in all lysates. | Western blot analysis of Rat Brain cell lysates showing detection of 53.4 kDa ULK3 protein using Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 53.4 kDa. Rabbit Anti-ULK3 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-642) | Rabbit Anti-ULK3 Antibody used in Western blot (WB) on A549 cell lysates (SPC-642) | Rabbit Anti-ULK3 Antibody used in Western blot (WB) on Brain cell lysates (SPC-642)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    ULK3 ATTO 700
    • Gene info
  • Gene symbol
    ULK3
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- ULK3 Polyclonal: ATTO 700
  • label filter
    • ATTO 700
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies