• Catalog number
    SPC-118D-RPE
  • Product name
    Rabbit Anti-Human SOD (Mn) Polyclonal: RPE
  • Size
    100 µg
  • Label
    RPE
  • Immunogen
    Human Mn SOD
  • Antibody s target
    Human SOD2
  • Antibody s full description
    Rabbit Anti-Human SOD (Mn) Polyclonal
  • Primary research fields
    Cancer, Oxidative Stress, Cell Signaling, Neuroscience, Neurodegeneration, Cardiovascular System
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Manganese SOD Antibody, IPO B Antibody, Mn SOD Antibody, SOD2 Antibody
  • Verified applications
    WB, IHC, IP, ELISA
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Rat, Mouse, Bovine, Dog, Chicken, Gerbil, Guinea Pig (Cavia porcellus), Pig, Hamster, Monkey, Rabbit, Sheep, African clawed frog (Xenopus laevis), Frog, Wood Frog (Rana sylvatica)
  • Antibody s recommended dilutions for use
    WB (1:5000), IHC (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~25kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    0.2 µg/ml of SPC-118 was sufficient for detection of Mn SOD in 20 µg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-mouse IgG:AP as the secondary antibody.
  • Antibody in cell
    Mitochondrion, Mitochondrion Matrix
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
  • Bibliography
    1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa Y., O’Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194 7. Wispe J.R., et al. (1989) BBA. 994: 30-36. 8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.
  • Released date
    1/Jun/2007
  • NCBI number
    NP_000627.2
  • Gene number
    6648
  • Protein number
    P04179
  • PubMed number
    25316288
  • Tested applications
    Western Blot
  • Tested reactivity
    North American wood frog (Rana sylvatica)
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunohistochemistry analysis using Rabbit Anti-SOD2 Polyclonal Antibody (SPC-118). Tissue: muscle fibres. Species: Rat. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (SPC-118) at 1:100. Left: Untreated, Right: treated with 3mmol*kg-1 NAC. Courtesy of: E. Barreiro, IMIM, Spain.. Rabbit Anti-SOD2 Antibody used in Immunohistochemistry (IHC) on Rat muscle fibres (SPC-118)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
  • Gene target
    SOD Mn RPE
  • Gene info
    Gene info
  • Gene symbol
    RPE, SOD3
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- SOD (Mn) Polyclonal: RPE
  • label filter
    • RPE
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies