Mouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa, Clone 1G10.H8
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Catalog numberSMC-150S
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Price:
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Size12 µg
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Clone1G10.H8
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ImmunogenFull length protein yeast HSP40 (YDJ1)
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Antibody s full descriptionMouse Anti-Yeast HSP40 Monoclonal IgG1 Kappa Antibody, Clone: 1G10.H8: Unconjugated
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameDNAJA2 Antibody, CPR3 Antibody, HIRIP4 Antibody, DNAJ Antibody, DNJ3 Antibody, DJ3 Antibody, RDJ2 Antibody, HIRa interacting protein4 Antibody
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Raised inMouse
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Antibody s targetHSP40
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Primary research fieldsCancer, Heat Shock
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BrandnameNone
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Antibodies applicationsWB, IP, ELISA
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Antibody s reactivityYeast
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Antibody s dilutionsWB (1:2000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storage50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~40kDa. Yeast specific product. Does not cross react with Human, Mouse or Rat.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis0.5 µg/ml of SMC-150 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm , Nucleus
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextHuman HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of HSP40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (5, 6). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).
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Bibliography1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36. 2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316. 3. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095. 4. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830. 5. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878. 6. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931. 7. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890.
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Released date1-Nov-2008
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_014335.1
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Gene number855661
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Protein numberP25491
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Antibody s datasheetContact our support service
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Representative figure legendWestern Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 1G10.H8 (SMC-150). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (SMC-150) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Hsp40 Antibody [1G10.H8] used in Western Blot (WB) on Yeast Cell lysates (SMC-150)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.012
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolDNAJB1
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Short nameMouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa, Clone 1G10 H8
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse, Ascomycota
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IsotypeIgG1 Kappa, IgG1
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LabelUnconjugated
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SpeciesYeast, Mouses, Yeasts
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Alternative nameMouse Antibody toYeast HSP40, YDJ1 monoclonal IgG1 Kappa, clonality 1G10.H8
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Alternative techniquemurine, antibodies
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Clone name1G10
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Gene info
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Identity
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Gene
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Long gene nameDnaJ heat shock protein family (Hsp40) member B1
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Synonyms gene
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Synonyms gene name
- DnaJ (Hsp40) homolog, subfamily B, member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-10-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DNAJ (HSP40) heat shock proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data