Mouse Anti-Mouse Synaptotagmin-7 Monoclonal IgG2B, Clone S275-14
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Catalog numberSMC-424D-A390
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Price:
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Size100 µg
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CloneS275-14
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ImmunogenFusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7
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Antibody s full descriptionMouse Anti-Mouse Synaptotagmin 7 Monoclonal IgG2B Antibody, Clone: S275-14: ATTO 390
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameIPCA-7 Antibody, PCANAP7 Antibody, Prostate cancer-associated protein 7 Antibody, SYT-7 Antibody, SYT7 Antibody, Synaptotagmin 7 Antibody, Synaptotagmin VII Antibody, SytVII Antibody
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Raised inMouse
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Antibody s targetSynaptotagmin 7
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Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~45kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at ~65kD.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-424 was sufficient for detection of Synaptotagmin-7 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasmic Vesicle
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextSynaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacylbackbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
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Bibliography1. Schengrund C.L., et al. (2002) J Biol Chem. 277: 32815. 2. Reichardt L.F., et al. (1981) J Cell Biol. 91:257.
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Released date28-Feb-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_061271.1
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Gene number54525
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Protein numberQ9R0N7
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-7 Monoclonal Antibody, Clone S275-14 (SMC-424). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-7 Monoclonal Antibody (SMC-424) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Synaptotagmin-7 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of Synaptotagmin 7 protein using Mouse Anti-Synaptotagmin 7 Monoclonal Antibody, Clone S275-14 (SMC-424). Primary Antibody: Mouse Anti-Synaptotagmin 7 Monoclonal Antibody (SMC-424) at 1:100, 1:250, 1:500, and 1:1000. Mouse Anti-Synaptotagmin-7 Antibody [S275-14] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-424) Mouse Anti-Synaptotagmin 7 Antibody [S275-14] used in Western Blot (WB) on Rat brain lysates (SMC-424)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolPIRC60, IGHV3OR16-14, SNORD114-14
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Short nameMouse Anti-Mouse Synaptotagmin-7 Monoclonal IgG2B, Clone S275-14
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2B, IgG2b
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LabelATTO 390
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Synaptotagmin-7 monoclonal IgG2B, clonality S275-14
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Alternative techniquemurine, antibodies
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Clone nameS275-14
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Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 60
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR16-14 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR16-14
- immunoglobulin heavy variable 3/OR16-14 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-14
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data