Mouse Anti-Mouse NrCAM Monoclonal IgG2a, Clone S364-51

  • Clone
    S364-51
  • Immunogen
    Fusion protein amino acids 30-845 (extracellular domain) of mouse NrCAM. Rat: 96% identity (795/822 amino acids identical). Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin.
  • Antibody s full description
    Mouse Anti-Mouse NrCAM Monoclonal IgG2a Antibody, Clone: S364-51: ATTO 680
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Neuronal cell adhesion molecule Antibody, Bravo Antibody, hBravo Antibody, KIAA0343 Antibody, MGC138845 Antibody, MGC138846 Antibody, Neuronal surface protein Bravo Antibody, Ng CAM related Antibody, Ng-CAM-related Antibody, NgCAM related cell adhesion molecule Antibody, NgCAM-related cell adhesion molecule Antibody, Nr CAM Antibody, Nr-CAM Antibody
  • Raised in
    Mouse
  • Antibody s target
    NrCAM
  • Primary research fields
    Neuroscience, Cell Structure, Membrane Markers
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~160kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-462 was sufficient for detection of NrCAM in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2.
  • Released date
    29-Oct-2013
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001139503.1
  • Gene number
    319504
  • Protein number
    Q810U4
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NrCAM Monoclonal Antibody, Clone S364-51 (SMC-462). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody (SMC-462) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NrCAM Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51 (SMC-462). Load: 10 µg. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody (SMC-462) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa. Mouse Anti-NrCAM Antibody [S364-51] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-462) Mouse Anti-NrCAM Antibody [S364-51] used in Western Blot (WB) on Rat Brain Membrane (SMC-462)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    IGHV8-51-1, IGHVII-51-2, IGLV1-51, IGHV5-51, ZNF3, SAMM50, OR7E26P
  • Short name
    Mouse Anti-Mouse NrCAM Monoclonal IgG2a, Clone S364-51
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG2a, IgG2a
  • Label
    ATTO 680
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Antibody toMouse neuronal cellular adhesion molecule monoclonal IgG2a, clonality S364-51
  • Alternative technique
    murine, antibodies
  • Alternative to gene target
    neuronal cell adhesion molecule, NRCAM and IDBG-36081 and ENSG00000091129 and 4897, protein binding involved in heterotypic cell-cell adhesion, Cell surfaces, Nrcam and IDBG-137217 and ENSMUSG00000020598 and 319504, NRCAM and IDBG-632519 and ENSBTAG00000006732 and 534500
  • Clone name
    S364-51
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable IGHV8-51-1 (non-functional)
  • Synonyms gene
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-51-1
    • immunoglobulin heavy variable (III)-51-1 pseudogene
    • immunoglobulin heavy variable (III)-51-1 (pseudogene)
    • immunoglobulin heavy variable IGHV8-51-1 (pseudogene)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (II)-51-2 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (II)-51-2
    • immunoglobulin heavy variable (II)-51-2 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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Mouse Anti Mouse NrCAM Monoclonal IgG2a Clone S364 51
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