Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4.
Antibody s full description
Mouse Anti-Mouse LGI1 Monoclonal IgG2a Antibody, Clone: S283-7: PerCP
Antibody s category
Antibody s other name
ADLTE Antibody, ADPAEF Antibody, ADPEAF Antibody, Epitempin 1 Antibody, EPT Antibody, ETL1 Antibody, IB1099 Antibody, leucine rich glioma inactivated 1 Antibody, OTTHUMP00000020121 Antibody, OTTHUMP00000020122 Antibody
Antibody s target
Primary research fields
Neuroscience, Neurodegeneration, Cell Signaling, Epigenetics, Cancer
WB, IHC, ICC/IF
Antibody s reactivity
Human, Mouse, Rat
Antibody s dilutions
WB (1:1000); optimal dilutions for assays should be determined by the user.
Protein G Purified
Antibody buffer for storage
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Antibody s concentration
Antibody s specificity
Blue Ice or 4ºC
Antibody certificate of analysis
1 µg/ml of SMC-461 was sufficient for detection of LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody in cell
Cell Junction , Golgi Apparatus , Endoplasmic Reticulum
See included datasheet or contact our support service
The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist; this LGI1 antibody will recognize only the longer form.
1. Chernova O.B., Somerville R.P. and Cowell J.K. (1998) Oncogene. 17:2873-81. 2. Fialka F., et al. (2008) Oral Oncol. 3. Gu W., et al. (2005) Mol. Biol. Evol. 22:2209-16. 4. Fukata Y., et al. (2006) Science. 313:1792-5.
To be tested
To be tested
Antibody s datasheet
Contact our support service
Representative figure link
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LGI1 Monoclonal Antibody, Clone S283-7 (SMC-461). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody (SMC-461) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LGI1 Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa LGI1 protein using Mouse Anti-LGI1 Monoclonal Antibody, Clone S283-7 (SMC-461). Load: 10 µg. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody (SMC-461) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa. Mouse Anti-LGI1 Antibody [S283-7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-461) Mouse Anti-LGI1 Antibody [S283-7] used in Western Blot (WB) on Rat Brain Membrane (SMC-461)
Country of production
Total weight kg
Net weight g
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
PIRC34, LGI1, PIRC33, PIRC32, SNORD114-7, IGHV3OR15-7, SNORD115-7, RNU6-7, PIRC30, PIRC31
Mouse Anti-Mouse LGI1 Monoclonal IgG2a, Clone S283-7
anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
Mouse Antibody toMouse LGI1 monoclonal IgG2a, clonality S283-7