gentaur logogentaur logo
x

Mouse Anti-Human Syntaxin Monoclonal IgG1, Clone SP-6

  • Catalog number
    SMC-181D-A680
  • Product name
    Mouse Anti-Human Syntaxin Monoclonal IgG1, Clone SP-6
  • Size
    100 µg
  • Label
    ATTO 680
  • Clone
    SP-6
  • Immunogen
    Raised against synaptic vesicle-containing fractions of immunoprecipitated human brain homogenate
  • Antibody s full description
    Mouse Anti-Human Syntaxin Monoclonal IgG1 Antibody, Clone: SP-6: ATTO 680
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    HPC1 Antibody, P35 1 Antibody, STX1 Antibody, STX1A Antibody, STX1B Antibody, STX2 Antibody, STX3 Antibody
  • Raised in
    Mouse
  • Isotype
    IgG1, IgG1
  • Antibody s target
    Syntaxin
  • Primary research fields
    Neuroscience, Cell Structure, Pre-Synaptic Markers, Cell Signaling, Trafficking, Cancer
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF
  • Antibody s reactivity
    Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~38kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-181 was sufficient for detection of Syntaxin in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Cell Junction , Synapse , Cell Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Syntax ins are a family of membrane integrated Q-SNARE proteins participating in exocytosis (1). They possess a single C-terminal transmembrane domain, a SNARE domain, and an N-terminal regulatory domain. This SNARE domain binds to synaptobrevin and SNAP-25 to form the SNARE complex (2, 3). Support of syntaxin’s involvement in vesicular transport and neurotransmission includes the requirement of its yeast homologs, SSO 1 and 2, in targeting and fusion of Golgi-derived vesicles with the plasma membrane (4). Syntaxin also provides the target pf clostridial neurotoxin C1, which blocks neurotransmitter release (5). The formation of 20S complex involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, a-SNAP and NSF (6).
  • Bibliography
    1. Bennett M.K., et al. (1993) Cell. 74(5): 863-873. 2. Walter A.M., Wiederhold K., Bruns D., Fasshaurer D. and Sorensen J.B. (2010) J Cell BIol. 188(3): 401-413. 3. Honma S., Taki K., Lei S., and Wakisaka S. (2010) Anat Rec. Epub. 4. Aalto M.K., Ronne H. and Keranen S. (1993) EMBO J. 12: 4095-4104. 5. Blasi J., et al. (1993) EMBO J. 12: 4821-4828. 6. Sollner T., et al. (1993) Cell. 75: 409-418.
  • Released date
    30-Jun-2010
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_004594.1
  • Gene number
    6804
  • Protein number
    Q16623
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunohistochemistry analysis using Mouse Anti-Syntaxin Monoclonal Antibody, Clone SP-6 (SMC-181). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Syntaxin Monoclonal Antibody (SMC-181) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle positive, basal layer staining in the epidermis and positive staining in the hair follicles. Western Blot analysis of Rat tissue lysate showing detection of Syntaxin protein using Mouse Anti-Syntaxin Monoclonal Antibody, Clone SP-6 (SMC-181). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Syntaxin Monoclonal Antibody (SMC-181) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Syntaxin Antibody [SP-6] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-181) Mouse Anti-Syntaxin Antibody [SP-6] used in Western Blot (WB) on Rat tissue lysate (SMC-181)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    Syntaxin Monoclonal IgG1 SP-6
    • Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    SFTPA1, SP6, PHF7, REXO5, SPATA1, SPESP1, SFTA2, SFTPB, SFTPA2, FDCSP
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse Anti- Syntaxin Monoclonal IgG1, Clone SP-6
  • label filter
    • ATTO 680
  • Clone name
    SP-6
  • technique filter
    • anti-Human
    • anti-
    • Mouse
    • anti
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies