Mouse Anti-Human p38 alpha (MAP Kinase) Monoclonal IgG1, Clone 9F12
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Catalog numberSMC-152D-RPE
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Price:
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Size100 µg
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Clone9F12
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ImmunogenFull length recombinant protein expressed in E.coli cells
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Antibody s full descriptionMouse Anti-Human p38 MAPK Monoclonal IgG1 Antibody, Clone: 9F12: RPE
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameCSAID Binding protein 1 Antibody, CSBP1 Antibody, CSBP2 Antibody, EXIP Antibody, MAP kinase MXI2 Antibody, MAPkinase p38alpha Antibody, MAPK14 Antibody, p38 ALPHA Antibody, p38 MAP kinase Antibody, p38 mitogen activated protein kinase Antibody, RK Antibody, SAPK 2A Antibody, Stress activated protein kinase 2A Antibody
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Raised inMouse
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Antibody s targetp38 MAPK
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Primary research fieldsCell Signaling, Post-translational Modifications, Phosphorylation, Cancer
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BrandnameNone
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Antibodies applicationsWB, IHC, IP, ELISA, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~38kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisDetects ~38kDa protein corresponding to p38α MAPK when loaded with 6 ng of purified p38α by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm , Nucleus
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).
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Bibliography1. Pearson, G. et al (2001). Endocrine Reviews 22 (2): 153-183. 2. Fan, Y. et al (2007) Mol. Cells 23 (1): 30-38. 3. Han, J. et al. (1994) Science 265: 808-811. 4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell. 115: 61-70. 6. Salojin KV, et al. (2006) J Immunol. 176 (3):1899-907. 7. Medicherla S. et al. (2006). J Pharmacol Exp Ther.318(1): 99-107.
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001306.1
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Gene number1432
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Protein numberQ16539
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (SMC-152). Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (SMC-152) at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute. Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (SMC-152). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (SMC-152) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. Western Blot analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (SMC-152). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (SMC-152) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-p38 MAPK Antibody [9F12] used in Immunohistochemistry (IHC) on Mouse Retinal Injury Model (SMC-152) Mouse Anti-p38 MAPK Antibody [9F12] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-152) Mouse Anti-p38 MAPK Antibody [9F12] used in Western Blot (WB) on Human Cell lysates (SMC-152)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThe Mouse Anti- p38 alpha (MAP Kinase) Monoclonal IgG1, Clone 9F12 is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolMOAP1, MAP7, MASP2, MASP1
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Short nameMouse Anti- p38 alpha (MAP Kinase) Monoclonal IgG1, Clone 9F12
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelRPE
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman p38 a (mitogen-stimulated protein kinase phosphorylation catalyst) monoclonal IgG1, clonality 9F12
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Alternative techniquemurine, antibodies
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Clone name9F12
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Gene info
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Identity
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Gene
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Long gene namemodulator of apoptosis 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-11-13
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Entrez gene record
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Pubmed identfication
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Classification
- Paraneoplastic Ma antigens
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrotubule associated protein 7
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameMBL associated serine protease 2
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Synonyms gene
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Synonyms gene name
- mannan-binding lectin serine protease 2
- mannan-binding lectin serine peptidase 1 pseudogene 1
- mannan-binding lectin serine protease 1 pseudogene 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-12-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Sushi domain containing
- Complement system activation components
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameMBL associated serine protease 1
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Synonyms gene
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Synonyms gene name
- mannan-binding lectin serine protease 1 (C4/C2 activating component of Ra-reactive factor)
- mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
- mannan binding lectin serine peptidase 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-07-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serine proteases
- Complement system activation components
- Sushi domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data