- Catalog numberSMC-145D-P594
- Product nameMouse Anti-Human HSP40, Hdj1 Monoclonal IgG1, Clone 3B9.E6
- Size100 µg
- PriceAsk For Price
- LabelPE/ATTO 594
- Clone3B9.E6
- ImmunogenRecombinant Protein HSP40 (Hdj1)
- Antibody s full descriptionMouse Anti-Human HSP40 Monoclonal IgG1 Antibody, Clone: 3B9.E6: PE/ATTO 594
- Antibody s categoryMonoclonal Antibodies
- Antibody s other nameDNAJ1 Antibody, NDAJB1 Antibody, HDJ1 Antibody, HSP40 Antibody, HSPF1 Antibody, DnaJ homolog subfamily B member 1 Antibody, Dna J protein homolog 1 Antibody, Heat shock 40 kDa protein 1 Antibody, HSP40 Antibody, heat shock protein 40 Antibody, Human DnaJ protein 1 Antibody, hDj-1 Antibody
- Raised inMouse
- IsotypeIgG1, IgG1
- Antibody s targetHSP40
- Primary research fieldsCancer, Heat Shock
- BrandnameNone
- Antibodies applicationsWB, IHC, ICC/IF, IP, ELISA
- Antibody s reactivityHuman, Mouse, Rat
- Antibody s dilutionsWB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
- PurityProtein G Purified
- Antibody buffer for storagePBS pH7.2, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~40kDa. Does not cross-react with HDJ2 or YDJ1.
- Storage recommendations-20ºC
- Shipping recommendationsBlue Ice or 4ºC
- Antibody certificate of analysis0.5 µg/ml of SMC-145 was sufficient for detection of HSP40 (HDJ1) in 15 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Antibody in cellCytoplasm , Nucleus
- Tissue specificitySee included datasheet or contact our support service
- Scientific contextHuman HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
- Bibliography1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36. 2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316. 3. Sohn S.Y., Kim S.B., Kim J., and Ahn B.Y. (2006) J Gen Virol. 87(7): 1883-91. 4. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878. 5. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931. 6. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890. 7. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095. 8. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
- Released date1-Nov-2008
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- NCBI numberNP_006136.1
- Gene number3337
- Protein numberP25685
- Antibody s datasheetContact our support service
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (SMC-145). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (SMC-145) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (SMC-145). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (SMC-145) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Hsp40 Antibody [3B9.E6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-145) Mouse Anti-Hsp40 Antibody [3B9.E6] used in Western Blot (WB) on Human Cell lysates (SMC-145)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilityIn Stock
- Other related products
- Gene targetHSP40 Hdj1 Monoclonal IgG1 3B9 E6
- Identity:HGNC:5270
- Gene:DNAJB1
- Long gene name:DnaJ heat shock protein family (Hsp40) member B1
- Synonyms gene name:DnaJ (Hsp40) homolog, subfamily B, member 1
- Synonyms:Hsp40Sis1RSPH16B
- Discovery year:1996-10-02
- Entrez gene record:3337
- Pubmed identification:8975727, 8250930
- RefSeq identity:NM_006145
- Classification:DNAJ (HSP40) heat shock proteins
- VEGA ID:OTTHUMG00000183289
- Gene symbolDNAJB1
- Hostmouse
- isotype filter
- IgG1
- Short nameMouse Anti- HSP40, Hdj1 Monoclonal IgG1, Clone 3B9 E6
- label filter
- PE ATTO 594
- Clone name3B9
- technique filter
- anti-Human
- anti-
- Mouse
- anti
- Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
- Alternative techniquemurine, antibodies