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Mouse Anti-Dibromo-tyrosine (DiBrY) Monoclonal IgG1, Clone 9F12

  • Catalog number
    SMC-507D
  • Product name
    Mouse Anti-Dibromo-tyrosine (DiBrY) Monoclonal IgG1, Clone 9F12
  • Size
    100 µg
  • Label
    Unconjugated
  • Clone
    9F12
  • Immunogen
    Synthetic 3,5-Dibromotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
  • Antibody s full description
    Mouse Anti- Dibromo-tyrosine Monoclonal IgG1 Antibody, Clone: 9F12: Unconjugated
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    3,5-Dibromo-tyrosine Antibody, 3,5-dibromotyrosine Antibody, Dibromotyrosine Antibody, Dibromo-tyrosine Antibody, DiBrY Antibody, Dibromotyrosine (DiBrY) Antibody, Dibromo-Tyrosine (DiBrY) Antibody, Dibromo-tyrosine [DiBrY] Antibody
  • Raised in
    Mouse
  • Isotype
    IgG1, IgG1
  • Antibody s target
    Dibromo-tyrosine
  • Primary research fields
    Cancer, Oxidative Stress, Protein Oxidation, Cell Signaling, Post Translational Modifications, Oxidation
  • Brandname
    MOLECULAR SIGNATURE®
  • Antibodies applications
    WB, ICC/IF, ELISA
  • Antibody s reactivity
    Species Independent
  • Antibody s dilutions
    WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Specific for 3,5-Dibromo-tyrosine. Does not cross-react with Nitrotyrosine.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:1000 dilution of SMC-507 was sufficient for detection of 3,5-Dibromotyrosine in 1 µg of 3, 5-Dibromotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
  • Antibody in cell
    See included datasheet or contact our support service
  • Tissue specificity
    See included datasheet or contact our support service
  • Released date
    23-Mar-2017
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    Refer to Swiss-Prot
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 9F12 (SMC-507). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody (SMC-507) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A,C,E,F) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain (C,D) Phalloidin Alex Fluor 633 F-Actin stain (E,F) Dibromo-tyrosine Antibody (G,F) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot analysis of 3, 5-Dibromotyrosine-BSA Conjugate showing detection of 67 kDa 3,5-Dibromotyrosine-BSA using Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody, Clone 9F12 (SMC-507). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: Nitrosylated-BSA. Lane 4: Dibromotyrosine-BSA. Load: 1 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody (SMC-507) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of 3,5-Dibromotyrosine-BSA using Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody, Clone 9F12 (SMC-507). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-3,5-Dibromotyrosine Monoclonal Antibody (SMC-507) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Mouse Anti-Dibromo-tyrosine Antibody [9F12] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Embryonic kidney cells (HEK293) (SMC-507) Mouse Anti-Dibromo-tyrosine Antibody [9F12] used in Western Blot (WB) on 3, 5-Dibromotyrosine-BSA Conjugate (SMC-507) Mouse Anti-Dibromo-tyrosine Antibody [9F12] used in Western Blot (WB) on Cervical Cancer cell line (HeLa) (SMC-507)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    Dibromo-tyrosine DiBrY Monoclonal IgG1 9F12
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse Anti-Dibromo-tyrosine (DiBrY) Monoclonal IgG1, Clone 9F12
  • label filter
    • Unconjugated
  • Clone name
    9F12
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies