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Single-Cell RNA Sequencing (scRNA-seq) is a revolutionary technique that allows researchers to analyze gene expression at the single-cell level. Unlike bulk RNA sequencing, which provides an average signal from thousands of cells, scRNA-seq uncovers the unique transcriptomic profiles of individual cells, enabling the discovery of rare cell populations, dynamic states, and cell-specific functions. This advanced technology is driving breakthroughs in developmental biology, cancer research, immunology, and beyond.