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Single-Cell ATAC Sequencing (scATAC-seq) is a cutting-edge technique that enables researchers to analyze chromatin accessibility in individual cells. By studying open chromatin regions, scATAC-seq provides insights into gene regulation, cell identity, and epigenetic mechanisms at an unprecedented resolution. This powerful method is transforming our understanding of cellular heterogeneity and the regulatory landscape in health and disease, offering new possibilities in developmental biology, cancer research, and beyond.