Gene Sequencing & Analysis

Gentaur provides custom viral vector production services for a wide range of research applications, including lentivirus, AAV, retrovirus, and adenovirus vectors. We deliver high-quality, titrated, research-grade viral particles with full quality control and tailored production scales.

Overview

Viral vectors are powerful tools for gene delivery, functional genomics, cell engineering, and gene therapy research. Gentaur’s production service supports custom packaging of your genetic constructs into robust, high-titer viral particles ready for transduction in mammalian systems. Our team leverages proprietary production systems, state-of-the-art facilities, and strict quality assurance protocols to ensure consistent results.

Service Highlights

1. Production of lentiviral, retroviral, AAV, and adenoviral vectors
2. High-titer virus particle manufacturing
3. Flexible production scales (small, medium, large)
4. BSL-2 compliant production and handling protocols
5. Comprehensive quality control (titer measurement, sterility checks)
6. Delivery of ready-to-use viral stocks

Supported Vector Types

Lentiviral Vectors

Efficient for stable gene delivery in dividing and non-dividing cells.

Retroviral Vectors

Ideal for stable integration into the host genome in dividing cells.

Adeno-Associated Virus (AAV)

Suitable for non-integrating gene delivery with low immunogenicity.

Adenoviral Vectors

High-capacity vectors for transient high-expression delivery.

Production Workflow

1. Sample Submission & Vector Assessment

Upload plasmid information and sequence details; confirm packaging compatibility.

2. Vector Preparation & Transfection

Preparation of delivery plasmids and helper constructs; optimized transfection.

3. Viral Particle Production & Harvesting

Culturing and collection of viral supernatant; multiple harvests if needed.

4. Purification & Concentration

Clarification and concentration of viral particles for high-titer yield.

5. Quality Control & Titering

Accurate viral titer measurement (functional or physical); optional sterility/mycoplasma checks.

6. Delivery & Documentation

Delivery of virus stocks with QC reports and handling recommendations.

Quality Control & Testing

Gentaur offers a range of QC testing options:

1. Functional titer determination (e.g., IFU/ml via qPCR)
2. Physical titer measurement (e.g., vg/ml)
3. Infectivity assessment
4. Sterility testing
5. Mycoplasma testing

(Specific QC options may vary by vector type and scale.)

Deliverables

When your production run is complete, you receive:

1. High-titer viral vector stock
2. Titer report (functional/physical)
3. QC summary
4. Handling & storage instructions
5. Sequence/construct documentation (if provided by client)

Turnaround & Options

1. Standard Production: ~10–15 business days
2. Express Production: ~7–10 business days (availability subject to workload)

Production time can vary based on vector type, scale, and QC depth.

Applications

1. Gene delivery for functional studies
2. Stable cell line generation
3. Gene knock-in/knock-out experiments (via integrative vectors)
4. Transduction of primary cells (depending on vector)
5. Protein overexpression studies
6. Pre-clinical research

Submission Requirements

Please prepare the following:

1. Plasmid DNA sequences (FASTA/GenBank)
2. Construct maps
3. Endotoxin-free plasmid DNA (recommended)
4. Packaging/transfer/helper plasmid information

(Vector design assistance available upon request)

Compliance Disclaimer

Research Use Only: Viral vector products are for research applications and not intended for clinical use or human therapeutic administration.