Professional laboratory testing and analysis services with state-of-the-art technology and expert scientific support.
Service Description
At Gentaur, we understand that precision is everything in molecular biology. That’s why we’ve developed a cutting-edge approach to producing custom DNA ladders tailored to your exact specifications. Using advanced techniques, we create size standards that are not only highly accurate and reproducible but also optimized for performance across a wide range of applications.
Rather than relying on conventional fragment assembly, our method ensures consistency and clarity in every band — delivering the confidence you need in your gel electrophoresis results.
At Gentaur, we don’t just mix DNA fragments — we use proven, state-of-the-art molecular biology methods to create high-quality, reliable DNA ladders designed just for your needs.
We start by amplifying your specified DNA fragments using high-fidelity DNA polymerases. This ensures the fragments have exact base pair lengths with minimal errors — critical for accurate sizing in your gels.
For complex or unusual fragment patterns, we use restriction enzymes or site-specific nucleases to cut plasmid DNA at precise locations. This lets us generate DNA fragments of non-standard sizes or unique patterns tailored to your requirements.
Each fragment is purified to remove primer dimers, nonspecific products, and residual enzymes. We use size-exclusion chromatography or gel extraction techniques to isolate clean, size-specific DNA bands, ensuring your ladder has sharp, distinct bands on gels.
Need fluorescent tags, biotin, or other chemical labels on your ladder fragments? We perform post-PCR labeling or incorporate modified nucleotides during amplification to give you ladders compatible with fluorescence imaging, affinity capture, or other detection methods.
Each fragment’s concentration is carefully measured using UV spectrophotometry and fluorometric assays. We then mix fragments at controlled ratios to produce ladders with optimized band intensities — so you get clear, easily interpretable patterns on your gels.
Before shipping, each batch undergoes:
