10X PCR Buffer with 15mM Mg2+

  • Catalog number
    41212
  • Price
    Please ask
  • Size
    4 x1.25 ml
  • Reagent properties
    Molecular biology DNA amplification enzymes and reagents
  • Description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Research sys.
  • Group
    PCR, polymerase chain reaction
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
  • Gene target
    10X   with   15mM   Mg2  
  • Short name
    10X PCR with 15mM Mg2+
  • Technique
    PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
  • Alternative name
    10X PCR test kit solution including 15mM Mg2+
  • Alternative technique
    dna-amplification
MeSH Data
  • Name
  • Concept
    Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
  • Tree numbers
    • E05.393.620.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
Filters
Contact
Chat with gentaur.com employee