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TM Yeast Pst1 Recombinant Protein

  • Catalog number
    95-151
  • Product name
    TM Yeast Pst1 Recombinant Protein
  • Size
    0.05 mg
  • About recombinant protein
    Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction (1). Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor (2). Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at around 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (Δoch1 Δmnn1 Δmnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified glycan-specific PGT antibodies (3). Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows high affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses (4).
  • Other name of the recombinant
    TM Yeast Pst1 Antibody: HPF2, HPF2, YDR055W, D4214, YD9609.09, Cell wall mannoprotein PST1, Haze protective factor 2
  • Recombinant in
    S. cerevisiae TM strain (Δoch1 Δmnn1 Δmnn4)
  • Activity
    not available
  • Use of recmbinant
    This recombinant protein can be used for WB, ELISA, and other assays to screen and identify mannose-specific antibodies. For research use only.
  • Protein is used in
    E, WB, MS
  • Suspention medium
    Lot specific, TBS or PBS
  • Keep at
    Store in working aliquots at -70˚C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
  • Rec PROTEIN GI
    17380259
  • Accession nr
    Q12355.1
  • KDa of reombinant
    100 kDa on SDS-PAGE
  • Pureity
    ~95%
  • About
    Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
  • Vial
    Liquid
  • NCBI ID
    851625
  • Gene symbol
    PST1
  • Gene name
    Protoplast secreted protein 1
  • Source
    Saccharomyces cerevisiae S288c
  • Uniprot
    Q12355
  • Pictures of recombinant
    Pictures_of_recombinant
  • Rec pdf
    Rec_pdf
  • Gene target
    TM Yeast Pst1 Protein
    • Gene info
  • Host
    Yeast, Ascomycota
  • Short name
    TM Yeast Pst1 Recombinant Protein
  • technique filter
    • Recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Alternative technique
    rec