M-CSF Recombinant Protein
Catalog number
40-606
Supplier
Price
376.27 USD
Size
0.01 mg
About recombinant protein
M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant human M-CSF is a 36.8 kDa homodimeric protein containing two 159 amino acid polypeptide subunits.
Other name of the recombinant
MCSF, CSF-1, Macrophage colony-stimulating factor 1, Lanimostim
Recombinant in
E. coli
His tag on the recombinant protein
no His tag
SEQUENCE
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
Activity
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is = 1.0 ng/mL, corresponding to a specific activity of = 1 x 106 units/mg.
Keep at
The lyophilized M-CSF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted M-CSF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing M-CSF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Rec PROTEIN GI
27262661
Accession nr
NP_000748.3
Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.
About
This product is for research use only.
Protein s origin
Human
Vial
Lyophilized
NCBI ID
1435
Gene name
colony stimulating factor 1 (macrophage)
Source
Homo sapiens
Uniprot
P09603
Additional source
Recombinants or rec. proteins
Group
recombinants
Gene target
Gene symbol
CSF1
Short name
M-CSF Recombinant Protein
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Alternative name
M-CSF Rec. Protein
Alternative technique
rec
Gene info
MeSH Data
Name
Concept
Scope note:The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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