Mucin-1 / MUC-1 (1036-1155) Recombinant Protein

• Background:

  Mucin-1 (MUC1) is also known as Tumor-associated epithelial membrane antigen (EMA), Polymorphic epithelial mucin (PEM), Peanut-reactive urinary mucin (PUM), PEMT, Krebs von den Lungen-6 (KL-6), CD antigen CD227, Episialin, H23AG. MUC1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs. Mucins protect the body from infection by pathogen binding to oligosaccharides in the extracellular domain, preventing the pathogen from reaching the cell surface. Except protective function by binding to pathogens, MUC1 also functions in a cell signaling capacity. Overexpression of MUC1 is often associated with colon, breast, ovarian, lung and pancreatic cancers.

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  7.2 kDa (partial α chain) & 8.2 kDa (partial β chain)

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The mature form of Mucin-1 is a non-covalent heterodimeric complex with the proteolytically cleaved partial α and partial β chain. Each partial α and partial β chain has a calculated MW of 7.2 kD

• Background Reference 01:

  Gendler SJ., et al., 1990, J. Biol. Chem. 265 (25) : 15286-93.

• Background Reference 02:

  Hollingsworth MA., et al., 2004, Nat. Rev. Cancer 4 (1) : 45-60.

• Background Reference 03:

  Moncada DM., et al., 2003, Trends Parasitol 19 (7) : 305-311.

• Background Reference 04:

  Singh PK., et al., 2006, Trends Cell Biol 16 (9) : 467-476.

• Other Product Names:

  Mucin 1, MUC1, CD227, EMA, H23AG, KL-6, MAM6, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM, CA 15-3, Episialin

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3