Coagulation factor XI / FXI Recombinant Protein

• Background:

  Coagulation factor XI is also known as F11, PTA and FXI. Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX. Factor XI is synthesized in the liver and circulates in the plasma as a disulfide bond-linked dimer complexed with high molecular weight kininogen. Selective cleavage of Arg-|-Ala and Arg-|-Val bonds in factor IX to form factor IXa. Factor XI is converted into XIa via either the contact phase of blood coagulation or thrombin-mediated activation on the platelet surface. The resulting XIa converts factor IX into IXa, which subsequently activates factor X into Xa.

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  25 mM HEPES, 150 mM NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  69.9 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The proprotein is activated by factor XIIa (or XII) and cleaved after Arg-387 into the light chain with calculated MW of 28.7 kDa, which contains the active site, and the heavy chain with calcula

• Background Reference 01:

  Gailani D, et al. J Thromb Haemost. 2009. Suppl 1: 75-8.

• Background Reference 02:

  Emsley J, et al. Blood. 2010. 115 (13) : 2569-77.

• Background Reference 03:

  Meijers J.C, et al. Biochemistry. 1998. 27:4231-4237.

• Other Product Names:

  Coagulation factor XI, FXI, PTA, F11

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3