Recombinant Escherichia coli 1, 4-dihydroxy-2-naphthoyl-CoA hydrolase (menI)

Recombinant Escherichia coli 1, 4-dihydroxy-2-naphthoyl-CoA hydrolase (menI)

• CAS Number: 9000-83-3
• Gene Name: menI
• UniProt: P77781
• Expression Region: 1-136aa
• Organism: Escherichia coli (strain K12)
• Target Sequence: MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDSRTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVCKPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL
• Tag: N-terminal 6xHis-SUMO-tagged
• Source: E.coli
• Field of Research: Others
• Assay Type: Developed Protein
• Relevance: Catalyzes the hydrolysis of 1, 4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA) to 1, 4-dihydroxy-2-naphthoate (DHNA). Also shows significant activity toward a wide range of acyl-CoA thioesters, and minimal activity toward benzoyl-holoEntB.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Full Length
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 27.9 kDa
• References & Citations: "Identification of a hotdog fold thioesterase involved in the biosynthesis of menaquinone in Escherichia coli." Chen M., Ma X., Chen X., Jiang M., Song H., Guo Z. J. Bacteriol. 195:2768-2775 (2013)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.