Human C3 (Complement Component 3) ELISA Kit

Human C3 (Complement Component 3) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein
• UniProt: P01024
• Field of Research: Signal transduction; Infection immunity; Immune molecule.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 20 µg/mL
• Sample Type: Serum, plasma and other biological fluids
• Detection Range: 0.32-20 µg/mL
• Sensitivity: 0.11 µg/mL