Anti-Alpha-1-Antichymotrypsin (SERPINA3) (Histiocytoma Marker) (AACT/1451 + AACT/1452)

Anti-Alpha-1-Antichymotrypsin (SERPINA3) (Histiocytoma Marker) (AACT/1451 + AACT/1452)

• Description: Alpha-1 Antichymotrypsin (AACT) is a plasma protease inhibitor synthesized in the liver as a single glycopeptide chain. In human, the normal serum level of AACT is about one-tenth that of alpha-1-antitrypsin (AAT), with which it shares nucleic acid and protein sequence homology. Both are major acute phase reactants; their concentrations in plasma increase in response to trauma, surgery and infection. Elevated levels of AACT are widely, but not universally, reported in the cerebrospinal fluid and plasma of AD patients. Prostate-specific antigen (PSA) and its SDS-stable complex with AACT are in widespread use as markers for the diagnosis of prostate cancer. AACT deficiency may also be a possible cause of chronic liver disease. AACT antibody reacts with histiocytes and histiocytic neoplasms. It is widely used to identify histiocytes and tumors derived from them. Acinar tumors of the pancreas and salivary gland may also exhibit AACT positivity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Alpha-1-antichymotrypsin; Serpin A3; SERPINA3; AACT; ACT; Cell growth-inhibiting gene 24/25 protein; Growth inhibiting protein 24; Growth inhibiting protein 25; Serine (or cysteine) proteinase inhibitor clade A member 3
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: SERPINA3
• Gene ID: 12
• NCBI Gene ID: 534293; 710488
• UniProt: P01011
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant human Antichymotrypsin (AACT) protein fragment (aa49-187) (exact sequence is proprietary)
• Target Antigen: Alpha-1 Antichymotrypsin
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: AACT/1451 AACT/1452
• Conjugation: Purified, BSA-free
• Disease: Tumor
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cancer
• Positive Control: HeLa Cells. Tonsil, Pancreas or Histiocytoma.
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 65-76 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Immunofluorescence 0.5-1 ug/mL Western blotting 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years