Anti-Clathrin, Light Chain (CLC/1421), Biotin conjugate

Anti-Clathrin, Light Chain (CLC/1421), Biotin conjugate

• Description: This antibody recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Clathrin light chain A; Clathrin light chain B; Clathrin light chain LCA; Clathrin light chain LCB; Clathrin light polypeptide A; Clathrin light polypeptide B; Clathrin, light polypeptide (Lca); Clathrin, light polypeptide (Lcb); CLTA; CLTB
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CLTA|CLTB
• Gene ID: 1211|1212
• NCBI Gene ID: 484241|522114
• UniProt: P09496|P09497
• Cellular Locus: Cytoplasmic|Lysosomes|Vesicular
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Purified recombinant N-terminal fragment of human Clathrin Light Chain
• Target Antigen: Clathrin, Light Chain
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: CLC/1421
• Conjugation: Biotin
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Membrane trafficking
• Positive Control: HeLa cells. Placenta or Prostate Carcinoma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 31-44 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years