HighYield T7 RNA Synthesis Kit RNA synthesis via in vitro transcription with T7 RNA Polymerase

HighYield T7 RNA Synthesis Kit RNA synthesis via in vitro transcription with T7 RNA Polymerase

• Description: HighYield T7 RNA Synthesis Kit is designed to produce large amounts of RNA via in vitro transcription with T7 RNA Polymerase. It is also suitable for the incorporation of modified nucleotides (e.g. Biotin- or fluorescent-labeled NTPs or cap analoga). The resulting RNA can subsequently be used for a variety of applications such as microinjection, transfection or in vitro translation experiments, structure/function studies or probes for in situ hybridization and Northern Blot experiments. The kit contains sufficient reagents for 50 reactions of 20 μl each (7.5 mM each NTP). A 20 μl reaction yields about 140-160 μg RNA after 30 min incubation (1 μg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).
• UNSPSC: 41100000
• UNSPSC Description: Laboratory and scientific equipment
• HS Code: 38221900
• eClass Number: 32160405
• eClass Product Group: Biochemical (Laboratory, Miscellaneous)
• Autoclavability: false
• Shipping Conditions: Shipped on gel packs.
• Storage Conditions: Store at -20 °C. Avoid freeze/thaw cycles.
• Shelf Life: 12 months after date of delivery