SCRIPT Direct RT-qPCR ProbesMaster Robust real-time RT-PCR master mix for highly sensitive and specific amplification directly from tissue, swabs or blood

SCRIPT Direct RT-qPCR ProbesMaster Robust real-time RT-PCR master mix for highly sensitive and specific amplification directly from tissue, swabs or blood

• Description: SCRIPT Direct RT-qPCR ProbesMaster is designed for quantitative real-time analysis of target RNA directly from animal- or plant tissue, swabs and blood. The mix allows robust amplification avoiding the requirement of any prior RNA purification procedures. The mix is recommended for use with dual-labeled fluorescent probes, e.g. TaqMan®, Molecular Beacon or Scorpion probes. It provides a powerful tool for multiplex-quantification of sample RNA in a broad dynamic range with exceptional sensitivity and precision. The mix contains all reagents required for RT-qPCR (except template, primer and labeled fluorescent probe) in a premixed 2 x concentrated ready-to-use solution. High robustness, reliability and sensitivity of the mix are based on a genetically engineered reverse transcriptase and an antibody-blocked hot start polymerase in combination with an optimized and well-balanced buffer system. The mix ensures fast and easy preparation with a minimum of pipetting steps and is specially recommended for: Direct amplification of target RNA from various tissues samples Direct detection of viral or bacterial RNA in nasal or throat swabs Direct PCR from blood samples Point-of-Care diagnostics Multiplexing Capability Real-time RT-PCR technology based on dual-labeled DNA probes provides a high sensitive and specific RT-PCR system with multiplexing capability. The simultaneous detection of multiple targets in a single tube requires a primer/probe set for each target amplification. Sequences and concentrations of primers and probes should be optimized to avoid mutual influence, secondary structures and primer-dimer formations. Amplification of each target is detected in a separate fluorescence channel.
• UNSPSC: 41106300
• UNSPSC Description: Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products
• HS Code: 35079090
• eClass Number: 32160502
• eClass Product Group: PCR Reagent (Laboratory)
• Concentration: 2x
• Form: Liquid
• Autoclavability: false
• Shipping Conditions: Shipped on gel packs.
• Storage Conditions: Store at -20 °C. Avoid freeze/thaw cycles. Stable at 4 °C for up to 4 weeks
• Shelf Life: 12 months