Pfu-X Polymerase Proofreading DNA polymerase for highest accuracy Pyrococcus furiosus, recombinant, E. coli

• Description:

  Pfu-X Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times.The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction but does not possess a 5'→3' exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-X Polymerase-generated PCR fragments are blunt-ended. The enzyme is highly purified and free of bacterial DNA. Fidelity of the enzyme:Pfu-X Polymerase is characterized by a 50-fold higher fidelity compared to Taq polymerase and a 2-fold higher fidelity compared to standard Pfu polymerase.ERPfu-X Polymerase = 0.25 x 10-6The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings(2d = amount of product / amount of template).

• CAS Number:

  9000-83-3

• UNSPSC:

  41106300

• UNSPSC Description:

  Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products

• HS Code:

  35079090

• eClass Number:

  32160502

• eClass Product Group:

  PCR Reagent (Laboratory)

• Concentration:

  2.5 units/μL

• Form:

  Liquid

• Autoclavability:

  false

• Shipping Conditions:

  Shipped on gel packs.

• Storage Conditions:

  Store at -20 °C. Avoid freeze/thaw cycles.

• Shelf Life:

  12 months